dc.contributor.author | Guo, Syuan-Ming | |
dc.contributor.author | Veneziano, Remi | |
dc.contributor.author | Gordonov, Simon | |
dc.contributor.author | Li, Li | |
dc.contributor.author | Danielson, Eric W | |
dc.contributor.author | Perez de Arce, Karen | |
dc.contributor.author | Park, Demian | |
dc.contributor.author | Kulesa, Anthony Benjamin | |
dc.contributor.author | Wamhoff, Eike-Christian | |
dc.contributor.author | Blainey, Paul C. | |
dc.contributor.author | Blainey, Paul C | |
dc.contributor.author | Boyden, Edward | |
dc.contributor.author | Cottrell, Jeffrey R. | |
dc.contributor.author | Bathe, Mark | |
dc.date.accessioned | 2020-06-29T18:36:36Z | |
dc.date.available | 2020-06-29T18:36:36Z | |
dc.date.issued | 2019-09 | |
dc.date.submitted | 2018-03 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/126016 | |
dc.description.abstract | Synapses contain hundreds of distinct proteins whose heterogeneous expression levels are determinants of synaptic plasticity and signal transmission relevant to a range of diseases. Here, we use diffusible nucleic acid imaging probes to profile neuronal synapses using multiplexed confocal and super-resolution microscopy. Confocal imaging is performed using high-affinity locked nucleic acid imaging probes that stably yet reversibly bind to oligonucleotides conjugated to antibodies and peptides. Super-resolution PAINT imaging of the same targets is performed using low-affinity DNA imaging probes to resolve nanometer-scale synaptic protein organization across nine distinct protein targets. Our approach enables the quantitative analysis of thousands of synapses in neuronal culture to identify putative synaptic sub-types and co-localization patterns from one dozen proteins. Application to characterize synaptic reorganization following neuronal activity blockade reveals coordinated upregulation of the post-synaptic proteins PSD-95, SHANK3 and Homer-1b/c, as well as increased correlation between synaptic markers in the active and synaptic vesicle zones. | en_US |
dc.description.sponsorship | National Institutes of Health (Award 1U01MH106011) | en_US |
dc.description.sponsorship | National Institutes of Health (Award R01-MH112694) | en_US |
dc.description.sponsorship | National Science Foundation (Grant 1305537) | en_US |
dc.description.sponsorship | National Science Foundation (Grant 1707999) | en_US |
dc.description.sponsorship | National Institute of Environmental Health Sciences (Grant P30-ES002109) | en_US |
dc.language.iso | en | |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/s41467-019-12372-6 | en_US |
dc.rights | Creative Commons Attribution 4.0 International license | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature | en_US |
dc.title | Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Guo, Syuang-Ming et al. "Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes." Nature Communications 10, 4377 (September 2019): 4377 © 2019 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Media Laboratory | en_US |
dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2019-12-10T14:35:58Z | |
dspace.date.submission | 2019-12-10T14:36:01Z | |
mit.journal.volume | 10 | en_US |
mit.journal.issue | 1 | en_US |
mit.metadata.status | Complete | |