High-definition spatial transcriptomics for in situ tissue profiling
Author(s)Vickovic, Sanja; Eraslan, Gökcen; Salmén, Fredrik; Klughammer, Johanna; Stenbeck, Linnea; Schapiro, Denis; Äijö, Tarmo; Bonneau, Richard; Bergenstråhle, Ludvig; Navarro, José Fernandéz; Gould, Joshua; Griffin, Gabriel K.; Borg, Åke; Ronaghi, Mostafa; Frisén, Jonas; Lundeberg, Joakim; Regev, Aviv; Ståhl, Patrik L.; ... Show more Show less
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Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Biology
Springer Science and Business Media LLC
Vickovic, Sanja et al. "High-definition spatial transcriptomics for in situ tissue profiling." Nature Methods 16, 10 (September 2019): 987–990 © 2019 The Author(s)
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