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dc.contributor.authorBushkin, G. Guy
dc.contributor.authorMorgan, Jeffrey T. (Jeffrey Thomas)
dc.contributor.authorRichardson, Kris
dc.contributor.authorLewis, Caroline
dc.contributor.authorChan, Sze Ham
dc.contributor.authorBartel, David
dc.contributor.authorFink, Gerald R
dc.date.accessioned2020-07-07T17:28:10Z
dc.date.available2020-07-07T17:28:10Z
dc.date.issued2019-07
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/1721.1/126068
dc.description.abstractDespite the vast number of modification sites mapped within mRNAs, known examples of consequential mRNA modifications remain rare. Here, we provide multiple lines of evidence to show that Ime4p, an N6-methyladenosine (m6A) methyltransferase required for meiosis in yeast, acts by methylating a site in the 3′ UTR of the mRNA encoding Rme1p, a transcriptional repressor of meiosis. Consistent with this mechanism, genetic analyses reveal that IME4 functions upstream of RME1. Transcriptome-wide, RME1 is the primary message that displays both increased methylation and reduced expression in an Ime4p-dependent manner. In yeast strains for which IME4 is dispensable for meiosis, a natural polymorphism in the RME1 promoter reduces RME1 transcription, obviating the requirement for methylation. Mutation of a single m6A site in the RME1 3′ UTR increases Rme1p repressor production and reduces meiotic efficiency. These results reveal the molecular and physiological consequences of a modification in the 3′ UTR of an mRNA.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM118153)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM108201)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM035010)en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionof10.1038/s41467-019-11232-7en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNatureen_US
dc.titlem⁶A modification of a 3′ UTR site reduces RME1 mRNA levels to promote meiosisen_US
dc.typeArticleen_US
dc.identifier.citationBushkin, G. Guy et al. “m⁶A modification of a 3′ UTR site reduces RME1 mRNA levels to promote meiosis.” Nature Communications, vol. 10, 2019, 3414 © 2019 The Author(s)en_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalNature Communicationsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-11-26T18:55:52Z
dspace.date.submission2019-11-26T18:55:54Z
mit.journal.volume10en_US
mit.metadata.statusComplete


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