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Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association

Author(s)
Monda, Julie Kathryn; Cheeseman, Iain M
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Creative Commons Attribution Noncommercial 3.0 unported license https://creativecommons.org/licenses/by-nc/3.0/
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Abstract
Nde1 is a key regulator of cytoplasmic dynein, binding directly to both dynein itself and the dynein adaptor, Lis1. Nde1 and Lis1 are thought to function together to promote dynein function, yet mutations in each result in distinct neurodevelopment phenotypes. To reconcile these phenotypic differences, we sought to dissect the contribution of Nde1 to dynein regulation and explore the cellular functions of Nde1. Here we show that an Nde1- Lis1 interaction is required for spindle pole focusing and Golgi organization but is largely dispensable for centrosome placement, despite Lis1 itself being required. Thus, diverse functions of dynein rely on distinct Nde1- and Lis1-mediated regulatory mechanisms. Additionally, we discovered a robust, isoform-specific interaction between human Nde1 and the 26S proteasome and identify precise mutations in Nde1 that disrupt the proteasome interaction. Together, our work suggests that Nde1 makes unique contributions to human neurodevelopment through its regulation of both dynein and proteasome function.
Date issued
2018-09
URI
https://hdl.handle.net/1721.1/126127
Department
Massachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Citation
Monda, Julie K. and Iain M. Cheeseman. "Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association." Molecular Biology of the Cell 29, 19 (September 2018): 2243-2357 © 2018 The Authors
Version: Final published version
ISSN
1059-1524
1939-4586

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