A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria
Author(s)Stokes, Jonathan; Gutierrez, Arnaud; Lopatkin, Allison J.; Andrews, Ian W.; French, Shawn; Matic, Ivan; Brown, Eric D.; Collins, James J.; ... Show more Show less
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Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity—an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.
DepartmentMassachusetts Institute of Technology. Institute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of Biological Engineering; Harvard University--MIT Division of Health Sciences and Technology
Springer Science and Business Media LLC
Stokes, J.M. et al. A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria. Nature Methods 16, 4 (March 2019): 303–306 © 2019 The Author(s)
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