Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification
Author(s)Santos, Maria C. Ferreira dos; Anderson, Cole P.; Neschen, Susanne; Zumbrennen-Bullough, Kimberly B.; Romney, Steven J.; Kahle-Stephan, Melanie; Rathkolb, Birgit; Gailus-Durner, Valerie; Fuchs, Helmut; Wolf, Eckhard; Rozman, Jan; de Angelis, Martin Hrabe; Cai, Weiling Maggie; Rajan, Malini; Hu, Jennifer; Dedon, Peter C; Leibold, Elizabeth A.; ... Show more Show less
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Regulation of cellular iron homeostasis is crucial as both iron excess and deficiency cause hematological and neurodegenerative diseases. Here we show that mice lacking iron-regulatory protein 2 (Irp2), a regulator of cellular iron homeostasis, develop diabetes. Irp2 post-transcriptionally regulates the iron-uptake protein transferrin receptor 1 (TfR1) and the iron-storage protein ferritin, and dysregulation of these proteins due to Irp2 loss causes functional iron deficiency in β cells. This impairs Fe–S cluster biosynthesis, reducing the function of Cdkal1, an Fe–S cluster enzyme that catalyzes methylthiolation of t6A37 in tRNALysUUU to ms2t6A37. As a consequence, lysine codons in proinsulin are misread and proinsulin processing is impaired, reducing insulin content and secretion. Iron normalizes ms2t6A37 and proinsulin lysine incorporation, restoring insulin content and secretion in Irp2−/− β cells. These studies reveal a previously unidentified link between insulin processing and cellular iron deficiency that may have relevance to type 2 diabetes in humans.
DepartmentMassachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Department of Biological Engineering
Springer Science and Business Media LLC
Ferreira dos Santos, Maria C. et al. "Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification." Nature Communications 11, 1 (January 2020): 296 © 2020 The Author(s)
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