Show simple item record

dc.contributor.authorErkekoglu, Ulfet Pinar
dc.contributor.authorChao, Ming-Wei
dc.contributor.authorTseng, Chia-Yi
dc.contributor.authorEngelward, Bevin P
dc.contributor.authorKose, Ozge
dc.contributor.authorKocer-Gumusel, Belma
dc.contributor.authorWogan, Gerald N
dc.contributor.authorTannenbaum, Steven R
dc.date.accessioned2020-07-21T19:29:24Z
dc.date.available2020-07-21T19:29:24Z
dc.date.issued2019-04
dc.date.submitted2018-06
dc.identifier.issn0004-1254
dc.identifier.urihttps://hdl.handle.net/1721.1/126291
dc.description.abstractExposure to alkyl anilines may lead to bladder cancer, which is the second most frequent cancer of the urogenital tract. 3,5-dimethylaniline is highly used in industry. Studies on its primary metabolite 3,5-dimethylaminophenol (3,5-DMAP) showed that this compound causes oxidative stress, changes antioxidant enzyme activities, and leads to death of different mammalian cells. However, there is no in vitro study to show the direct effects of 3,5-DMAP on human bladder and urothelial cells. Selenocompounds are suggested to decrease oxidative stress caused by some chemicals, and selenium supplementation was shown to reduce the risk of bladder cancer. The main aim of this study was to investigate whether selenocompounds organic selenomethionine (SM, 10 μmol/L) or inorganic sodium selenite (SS, 30 nmol/L) could reduce oxidative stress, DNA damage, and apoptosis in UROtsa cells exposed to 3,5-DMAP. 3,5-DMAP caused a dose-dependent increase in intracellular generation of reactive oxygen species, and its dose of 50 μmol/L caused lipid peroxidation, protein oxidation, and changes in antioxidant enzyme activities in different cellular fractions. The comet assay also showed single-strand DNA breaks induced by the 3,5-DMAP dose of 50 μmol/L, but no changes in double-strand DNA breaks. Apoptosis was also triggered. Both selenocompounds provided partial protection against the cellular toxicity of 3,5-DMAP. Low selenium status along with exposure to alkyl anilines can be a major factor in the development of bladder cancer. More mechanistic studies are needed to specify the role of selenium in bladder cancer.en_US
dc.language.isoen
dc.publisherWalter de Gruyter GmbHen_US
dc.relation.isversionofhttp://dx.doi.org/10.2478/aiht-2019-70-3159en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourceDe Gruyteren_US
dc.titleAntioxidants and selenocompounds inhibit 3,5-dimethylaminophenol toxicity to human urothelial cellsen_US
dc.typeArticleen_US
dc.identifier.citationErkekoglu, Pinar et al. "Antioxidants and selenocompounds inhibit 3,5-dimethylaminophenol toxicity to human urothelial cells." Archives of Industrial Hygiene and Toxicology (April 2019): 18-29 © 2019 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.relation.journalArchives of Industrial Hygiene and Toxicologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-03-05T18:06:14Z
dspace.date.submission2020-03-05T18:06:15Z
mit.journal.volume70en_US
mit.journal.issue1en_US
mit.licensePUBLISHER_CC
mit.metadata.statusComplete


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record