Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells
Author(s)
Kröger, Cornelia; Afeyan, Alexander; Mraz, Jasmin; Eaton, Elinor Ng; Reinhardt, Ferenc; Thiru, Prathapan; Bierie, Brian; Ye, Xin; Weinberg, Robert A; ... Show more Show less![Thumbnail](/bitstream/handle/1721.1/126342/7353.full.pdf.jpg?sequence=4&isAllowed=y)
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Carcinoma cells residing in an intermediate phenotypic state along the epithelial–mesenchymal (E–M) spectrum are associated with malignant phenotypes, such as invasiveness, tumor-initiating ability, and metastatic dissemination. Using the recently described CD104+/CD44hi antigen marker combination, we isolated highly tumorigenic breast cancer cells residing stably—both in vitro and in vivo—in an intermediate phenotypic state and coexpressing both epithelial (E) and mesenchymal (M) markers. We demonstrate that tumorigenicity depends on individual cells residing in this E/M hybrid state and cannot be phenocopied by mixing two cell populations that reside stably at the two ends of the spectrum, i.e., in the E and in the M state. Hence, residence in a specific intermediate state along the E–M spectrum rather than phenotypic plasticity appears critical to the expression of tumor-initiating capacity. Acquisition of this E/M hybrid state is facilitated by the differential expression of EMT-inducing transcription factors (EMT-TFs) and is accompanied by the expression of adult stem cell programs, notably, active canonical Wnt signaling. Furthermore, transition from the highly tumorigenic E/M state to a fully mesenchymal phenotype, achieved by constitutive ectopic expression of Zeb1, is sufficient to drive cells out of the E/M hybrid state into a highly mesenchymal state, which is accompanied by a substantial loss of tumorigenicity and a switch from canonical to noncanonical Wnt signaling. Identifying the gatekeepers of the various phenotypic states arrayed along the E–M spectrum is likely to prove useful in developing therapeutic approaches that operate by shifting cancer cells between distinct states along this spectrum.
Date issued
2019-04Department
Whitehead Institute for Biomedical Research; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Division of Comparative Medicine; Ludwig Center for Molecular Oncology (Massachusetts Institute of Technology)Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
Proceedings of the National Academy of Sciences
Citation
Kröger, Cornelia et al. “Acquisition of a hybrid E/M state is essential for tumorigenicity of basal breast cancer cells.” Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 15, 2019, pp. 7353-7362 © 2019 The Author(s)
Version: Final published version
ISSN
0027-8424