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dc.contributor.authorKaiser, Tobias
dc.contributor.authorFeng, Guoping
dc.date.accessioned2020-07-27T20:16:49Z
dc.date.available2020-07-27T20:16:49Z
dc.date.issued2019-07
dc.date.submitted2019-07
dc.identifier.issn2373-2822
dc.identifier.urihttps://hdl.handle.net/1721.1/126404
dc.description.abstractMicroglia are specialized brain-resident macrophages with important functions in health and disease. To improve our understanding of these cells, the research community needs genetic tools to identify and control them in a manner that distinguishes them from closely related cell types. We have targeted the recently discovered microglia-specific Tmem119 gene to generate knock-in mice expressing EGFP (JAX#031823) or CreERT2 (JAX#031820) for the identification and manipulation of microglia, respectively. Genetic characterization of the locus and qPCR-based analysis demonstrate correct positioning of the transgenes and intact expression of endogenous Tmem119 in the knock-in mouse models. Immunofluorescence analysis further shows that parenchymal microglia, but not other brain macrophages, are completely and faithfully labeled in the EGFP-line at different time points of development. Flow cytometry indicates highly selective expression of EGFP in CD11b+CD45lo microglia. Similarly, immunofluorescence and flow cytometry analyses using a Cre-dependent reporter mouse line demonstrate activity of CreERT2 primarily in microglia upon tamoxifen administration with the caveat of activity in leptomeningeal cells. Finally, flow cytometric analyses reveal absence of EGFP expression and minimal activity of CreERT2 in blood monocytes of the Tmem119-EGFP and Tmem119-CreERT2 lines, respectively. These new transgenic lines extend the microglia toolbox by providing the currently most specific genetic labeling and control over these cells in the myeloid compartment of mice.en_US
dc.description.sponsorshipNational Institute of Mental Health (Grant 5R01MH097104)en_US
dc.description.sponsorshipNational Cancer Institute (Grant P30-CA14051)en_US
dc.language.isoen
dc.publisherSociety for Neuroscienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1523/eneuro.0448-18.2019en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceSociety for Neurocienceen_US
dc.titleTmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microgliaen_US
dc.typeArticleen_US
dc.identifier.citationKaiser, Tobias and Guoping Feng. "Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microglia." eNeuro 6, 4 (July 2019): 0448-18.2019 © 2019 Kaiser and Fengen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.relation.journaleNeuroen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-01T12:50:28Z
dspace.date.submission2019-10-01T12:50:35Z
mit.journal.volume6en_US
mit.journal.issue4en_US


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