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dc.contributor.authorTam, Justina O.
dc.contributor.authorde Puig Guixe, Helena
dc.contributor.authorYen, Chun-wan
dc.contributor.authorBosch, Irene
dc.contributor.authorGomez-Marquez, Jose Zacapa
dc.contributor.authorClavet, Charles
dc.contributor.authorHamad-Schifferli, Kimberly
dc.contributor.authorGehrke, Lee
dc.date.accessioned2020-07-30T00:14:50Z
dc.date.available2020-07-30T00:14:50Z
dc.date.issued2017-01
dc.identifier.issn1532-1819
dc.identifier.issn1532-4230
dc.identifier.urihttps://hdl.handle.net/1721.1/126437
dc.description.abstractPoint-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.en_US
dc.language.isoen
dc.publisherInforma UK Limiteden_US
dc.relation.isversionofhttp://dx.doi.org/10.1080/15321819.2016.1269338en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleA comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassaysen_US
dc.typeArticleen_US
dc.identifier.citationTam, Justina O. et al. "A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays." Journal of Immunoassay and Immunochemistry 38, 4 (January 2017): 355-377 © 2017 Taylor & Francisen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentSUTD-MIT International Design Centre (IDC)en_US
dc.relation.journalJournal of Immunoassay and Immunochemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-09T13:52:17Z
dspace.date.submission2019-10-09T13:52:18Z
mit.journal.volume38en_US
mit.journal.issue4en_US


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