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dc.contributor.authorTekin, Halil
dc.contributor.authorSimmons, Sean
dc.contributor.authorCummings, Beryl
dc.contributor.authorGao, Linyi
dc.contributor.authorAdiconis, Xian
dc.contributor.authorHession, Cynthia C.
dc.contributor.authorGhoshal, Ayan
dc.contributor.authorDionne, Danielle
dc.contributor.authorChoudhury, Sourav
dc.contributor.authorYesilyurt, Volkan
dc.contributor.authorSanjana, Neville E
dc.contributor.authorShi, Xi
dc.contributor.authorLu, Congyi
dc.contributor.authorHeidenreich, Matthias
dc.contributor.authorPan, Jen Q.
dc.contributor.authorLevin, Joshua Z.
dc.contributor.authorZhang, Feng
dc.date.accessioned2020-08-05T18:19:23Z
dc.date.available2020-08-05T18:19:23Z
dc.date.issued2018-04
dc.date.submitted2017-05
dc.identifier.issn2157-846X
dc.identifier.urihttps://hdl.handle.net/1721.1/126476
dc.description.abstractUnderstanding neurological diseases requires tractable genetic systems, and engineered three-dimensional (3D) neural tissues are an attractive choice. Yet how the cellular transcriptomic profiles in these tissues are affected by the encapsulating materials and are related to the human brain transcriptome is not well understood. Here, we report the characterization of the effects of different culturing conditions on the transcriptomic profiles of induced neuronal cells and developed a method for the rapid generation of 3D co-cultures of neuronal and astrocytic cells from the same pool of human embryonic stem cells. By comparing the gene-expression profiles of neuronal cells in culture conditions relevant to the developing human brain, we found that modifying the degree of crosslinking of composite hydrogels can tune expression patterns so that they correlate with those of specific brain regions and developmental stages. Moreover, single-cell-sequencing results showed that our engineered tissues recapitulate transcriptional patterns of cell types in the human brain. Analyses of culturing conditions will inform the development of 3D neural tissues for use as tractable models of brain diseases.en_US
dc.description.sponsorshipNIH (Grants 1R01-HG009761, 1R01-MH110049 and 1DP1-HL141201)en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41551-018-0219-9en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleEffects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neuronsen_US
dc.typeArticleen_US
dc.identifier.citationTekin, Halil et al. "Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons." Nature Biomedical Engineering 2, 7 (April 2018): 540–554 © 2018 The Author(s)en_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.relation.journalNature Biomedical Engineeringen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-08T12:31:13Z
dspace.date.submission2019-10-08T12:31:15Z
mit.journal.volume2en_US
mit.journal.issue7en_US


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