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dc.contributor.authorBongrand, Clotilde
dc.contributor.authorMoriano-Gutierrez, Silvia
dc.contributor.authorArevalo, Philip Alexander
dc.contributor.authorMcFall-Ngai, Margaret
dc.contributor.authorVisick, Karen L.
dc.contributor.authorPolz, Martin F
dc.contributor.authorRuby, Edward G.
dc.date.accessioned2020-09-01T19:05:53Z
dc.date.available2020-09-01T19:05:53Z
dc.date.issued2020-03
dc.date.submitted2020-01
dc.identifier.issn2150-7511
dc.identifier.urihttps://hdl.handle.net/1721.1/126880
dc.description.abstractThe luminous marine Gram-negative bacterium Vibrio Aliivibrio fischeri is the natural light organ symbiont of several squid species, including the Hawaiian bobtail squid, Euprymna scolopes, and the Japanese bobtail squid, Euprymna morsei. Work with E. scolopes has shown how the bacteria establish their niche in the light organ of the newly hatched host. Two types of V. fischeri strains have been distinguished based upon their behavior in cocolonization competition assays in juvenile E. scolopes, i.e., (i) niche-sharing or (ii) niche-dominant behavior. This study aimed to determine whether these behaviors are observed with other V. fischeri strains or whether they are specific to those isolated from E. scolopes light organs. Cocolonization competition assays between V. fischeri strains isolated from the congeneric squid E. morsei or from other marine animals revealed the same sharing or dominant behaviors. In addition, whole-genome sequencing of these strains showed that the dominant behavior is polyphyletic and not associated with the presence or absence of a single gene or genes. Comparative genomics of 44 squid light organ isolates from around the globe led to the identification of symbiosis-specific candidates in the genomes of these strains. Colonization assays using genetic derivatives with deletions of these candidates established the importance of two such genes in colonization. This study has allowed us to expand the concept of distinct colonization behaviors to strains isolated from a number of squid and fish hosts. There is an increasing recognition of the importance of strain differences in the ecology of a symbiotic bacterial species and, in particular, how these differences underlie crucial interactions with their host. Nevertheless, little is known about the genetic bases for these differences, how they manifest themselves in specific behaviors, and their distribution among symbionts of different host species. In this study, we sequenced the genomes of <jats:named-content content-type="genus-species">Vibrio fischeri</jats:named-content> isolated from the tissues of squids and fishes and applied comparative genomics approaches to look for patterns between symbiont lineages and host colonization behavior. In addition, we identified the only two genes that were exclusively present in all <jats:named-content content-type="genus-species">V. fischeri</jats:named-content> strains isolated from the light organs of sepiolid squid species. Mutational studies of these genes indicated that they both played a role in colonization of the squid light organ, emphasizing the value of applying a comparative genomics approach in the study of symbioses.en_US
dc.description.sponsorshipNIGMS (Grants GM099507, GM114288, GM130355 and GM135254)en_US
dc.description.sponsorshipNIAID (Grant AI050661)en_US
dc.description.sponsorshipNational Institutes of Health, Office of the Director (Grant OD011024)en_US
dc.language.isoen
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1128/mbio.03407-19en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourcemBioen_US
dc.titleUsing Colonization Assays and Comparative Genomics To Discover Symbiosis Behaviors and Factors in Vibrio fischerien_US
dc.typeArticleen_US
dc.identifier.citationBongrand, Clotilde et al. "Using Colonization Assays and Comparative Genomics To Discover Symbiosis Behaviors and Factors in Vibrio fischeri." mBio 11, 2 (March 2020): e03407-19. © 2020 Bongrand et al.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.relation.journalmBioen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-06-01T17:10:30Z
dspace.date.submission2020-06-01T17:10:33Z
mit.journal.volume11en_US
mit.journal.issue2en_US
mit.licensePUBLISHER_CC


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