dc.contributor.author | Cahill, Lucas Christopher | |
dc.contributor.author | Wu, Yubo | |
dc.contributor.author | Yoshitake, Tadayuki | |
dc.contributor.author | Ponchiardi, Cecilia | |
dc.contributor.author | Giacomelli, Michael | |
dc.contributor.author | Wagner, Andrew A. | |
dc.contributor.author | Rosen, Seymour | |
dc.contributor.author | Fujimoto, James G | |
dc.date.accessioned | 2020-12-15T21:26:55Z | |
dc.date.available | 2020-12-15T21:26:55Z | |
dc.date.issued | 2019-11 | |
dc.date.submitted | 2019-10 | |
dc.identifier.issn | 0893-3952 | |
dc.identifier.issn | 1530-0285 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/128835 | |
dc.description.abstract | Intraoperative evaluation of specimens during radical prostatectomy using frozen sections can be time and labor intensive. Nonlinear microscopy (NLM) is a fluorescence microscopy technique that can rapidly generate images that closely resemble H&E histology in freshly excised tissue, without requiring freezing or microtome sectioning. Specimens are stained with nuclear and cytoplasmic/stromal fluorophores, and NLM evaluation can begin within 3 min of grossing. Fluorescence signals can be displayed using an H&E color scale, facilitating pathologist interpretation. This study evaluates the accuracy of prostate cancer detection in a blinded reading of NLM images compared with the gold standard of formalin-fixed, paraffin-embedded H&E histology. A total of 122 freshly excised prostate specimens were obtained from 40 patients undergoing radical prostatectomy. The prostates were grossed, dissected into specimens of ~10 × 10 mm with 1–4 mm thickness, stained for 2 min for nuclear and cytoplasmic/stromal contrast, and then rinsed with saline for 30 s. NLM images were acquired and multiple images were stitched together to generate large field of view, centimeter-scale digital images suitable for reading. Specimens were then processed for standard paraffin H&E. The study protocol consisted of training, pretesting, and blinded reading phases. After a washout period, pathologists read corresponding paraffin H&E slides. Three pathologists achieved a 95% or greater sensitivity with 100% specificity for detecting cancer on NLM compared with paraffin H&E. Pooled sensitivity and specificity was 97.3% (93.7–99.1%; 95% confidence interval) and 100.0% (97.0–100.0%), respectively. Interobserver agreement for NLM reading had a Fleiss κ = 0.95. The high cancer detection accuracy and rapid specimen preparation suggest that NLM may be useful for intraoperative evaluation in radical prostatectomy. | en_US |
dc.description.sponsorship | National Institutes of Health (Grants R01-CA178636-05, R01-CA075289-20, F32-CA183400-02) | en_US |
dc.description.sponsorship | Air Force Office of Scientific Research (Contracts FA9550-12-1-0551 and FA9550-15-1-0473) | en_US |
dc.language.iso | en | |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/s41379-019-0408-4 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | PMC | en_US |
dc.title | Nonlinear microscopy for detection of prostate cancer: analysis of sensitivity and specificity in radical prostatectomies | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Cahill, Lucas C. et al. "Nonlinear microscopy for detection of prostate cancer: analysis of sensitivity and specificity in radical prostatectomies." 33, 5 (November 2019): 916–923 © 2019 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Research Laboratory of Electronics | en_US |
dc.contributor.department | Harvard University--MIT Division of Health Sciences and Technology | en_US |
dc.relation.journal | Modern Pathology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2020-12-11T19:53:45Z | |
dspace.orderedauthors | Cahill, LC; Wu, Y; Yoshitake, T; Ponchiardi, C; Giacomelli, MG; Wagner, AA; Rosen, S; Fujimoto, JG | en_US |
dspace.date.submission | 2020-12-11T19:53:49Z | |
mit.journal.volume | 33 | en_US |
mit.journal.issue | 5 | en_US |
mit.license | PUBLISHER_POLICY | |
mit.metadata.status | Complete | |