dc.contributor.author | Giacomelli, Michael | |
dc.contributor.author | Faulkner-Jones, Beverly E. | |
dc.contributor.author | Cahill, Lucas Christopher | |
dc.contributor.author | Yoshitake, Tadayuki | |
dc.contributor.author | Do, Daihung | |
dc.contributor.author | Fujimoto, James G | |
dc.date.accessioned | 2021-01-05T21:44:53Z | |
dc.date.available | 2021-01-05T21:44:53Z | |
dc.date.issued | 2019-07 | |
dc.date.submitted | 2019-07 | |
dc.identifier.issn | 2156-7085 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/128963 | |
dc.description.abstract | Mohs surgery uses en face frozen section analysis (FSA) with complete margin examination for the excision of select basal cell carcinomas (BCC), obtaining excellent cosmetic outcomes and extremely low recurrence rates. However, Mohs with FSA is time-consuming because of the need to iteratively perform cryosectioning on sequential excisions. Fluorescent microscopies can image tissue specimens without requiring physical sectioning, potentially reducing the time to perform Mohs surgery. We demonstrate a protocol for nonlinear microscopy (NLM) imaging of surgical specimens that combines dual agent staining, virtual H&E rendering, and video rate imaging. We also introduce a novel protocol that enables micron-level co-registration of NLM images with FSA histology, and demonstrate that NLM can reproduce similar features similar to FSA in BCC specimens with both negative and positive surgical margins. We show that the fluorescent labels can be extracted with conventional vacuum infiltration processing, enabling subsequent immunohistochemistry on fluorescently labeled tissue. This protocol can also be applied to evaluate the performance of NLM compared with FSA in a wide range of pathologies for intraoperative consultation. | en_US |
dc.description.sponsorship | National Institutes of Health (Grants R01-CA075289, R01-CA178636) | en_US |
dc.description.sponsorship | Air Force Office of Scientific Research (Grant FA9550-15-1-0473) | en_US |
dc.language.iso | en | |
dc.publisher | Optical Society of America (OSA) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1364/boe.10.004249 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | OSA Publishing | en_US |
dc.title | Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Giacomelli, Michael G. et al. "Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins." Biomedical Optics Express 10, 8 (July 2019): 4249-4260 © 2019 Optical Society of America | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Research Laboratory of Electronics | en_US |
dc.relation.journal | Biomedical Optics Express | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2020-12-14T20:05:15Z | |
dspace.orderedauthors | Giacomelli, MG; Faulkner-Jones, BE; Cahill, LC; Yoshitake, T; Do, D; Fujimoto, JG | en_US |
dspace.date.submission | 2020-12-14T20:05:20Z | |
mit.journal.volume | 10 | en_US |
mit.journal.issue | 8 | en_US |
mit.license | PUBLISHER_POLICY | |
mit.metadata.status | Complete | |