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dc.contributor.authorGiacomelli, Michael
dc.contributor.authorFaulkner-Jones, Beverly E.
dc.contributor.authorCahill, Lucas Christopher
dc.contributor.authorYoshitake, Tadayuki
dc.contributor.authorDo, Daihung
dc.contributor.authorFujimoto, James G
dc.date.accessioned2021-01-05T21:44:53Z
dc.date.available2021-01-05T21:44:53Z
dc.date.issued2019-07
dc.date.submitted2019-07
dc.identifier.issn2156-7085
dc.identifier.urihttps://hdl.handle.net/1721.1/128963
dc.description.abstractMohs surgery uses en face frozen section analysis (FSA) with complete margin examination for the excision of select basal cell carcinomas (BCC), obtaining excellent cosmetic outcomes and extremely low recurrence rates. However, Mohs with FSA is time-consuming because of the need to iteratively perform cryosectioning on sequential excisions. Fluorescent microscopies can image tissue specimens without requiring physical sectioning, potentially reducing the time to perform Mohs surgery. We demonstrate a protocol for nonlinear microscopy (NLM) imaging of surgical specimens that combines dual agent staining, virtual H&E rendering, and video rate imaging. We also introduce a novel protocol that enables micron-level co-registration of NLM images with FSA histology, and demonstrate that NLM can reproduce similar features similar to FSA in BCC specimens with both negative and positive surgical margins. We show that the fluorescent labels can be extracted with conventional vacuum infiltration processing, enabling subsequent immunohistochemistry on fluorescently labeled tissue. This protocol can also be applied to evaluate the performance of NLM compared with FSA in a wide range of pathologies for intraoperative consultation.en_US
dc.description.sponsorshipNational Institutes of Health (Grants R01-CA075289, R01-CA178636)en_US
dc.description.sponsorshipAir Force Office of Scientific Research (Grant FA9550-15-1-0473)en_US
dc.language.isoen
dc.publisherOptical Society of America (OSA)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1364/boe.10.004249en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceOSA Publishingen_US
dc.titleComparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical marginsen_US
dc.typeArticleen_US
dc.identifier.citationGiacomelli, Michael G. et al. "Comparison of nonlinear microscopy and frozen section histology for imaging of Mohs surgical margins." Biomedical Optics Express 10, 8 (July 2019): 4249-4260 © 2019 Optical Society of Americaen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Research Laboratory of Electronicsen_US
dc.relation.journalBiomedical Optics Expressen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2020-12-14T20:05:15Z
dspace.orderedauthorsGiacomelli, MG; Faulkner-Jones, BE; Cahill, LC; Yoshitake, T; Do, D; Fujimoto, JGen_US
dspace.date.submission2020-12-14T20:05:20Z
mit.journal.volume10en_US
mit.journal.issue8en_US
mit.licensePUBLISHER_POLICY


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