PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function
Author(s)Wang, Yuanyuan; Kerrisk Campbell, Meghan; Tom, Irene; Foreman, Oded; Hanson, Jesse E.; Sheng, Morgan Hwa-Tze; ... Show more Show less
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The N-terminal domain (NTD) of the GluN1 subunit (GluN1-NTD) is important for NMDA receptor structure and function, but the interacting proteins of the GluN1-NTD are not well understood. Starting with an unbiased screen of ~ 1,500 transmembrane proteins using the purified GluN1-NTD protein as a bait, we identify Protocadherin 7 (PCDH7) as a potential interacting protein. PCDH7 is highly expressed in the brain and has been linked to CNS disorders, including epilepsy. Using primary neurons and brain slice cultures, we find that overexpression and knockdown of PCDH7 induce opposing morphological changes of dendritic structures. We also find that PCDH7 overexpression reduces synaptic NMDA receptor currents. These data show that PCDH7 can regulate dendritic spine morphology and synaptic function, possibly via interaction with the GluN1 subunit.
DepartmentBroad Institute of MIT and Harvard; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Springer Science and Business Media LLC
Wang, Yuanyuan et al. "PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function." Scientific Reports 10, 1 (July 2020): 10951. © 2020 The Author(s)
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