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dc.contributor.authorRaman, Ritu
dc.contributor.authorRousseau, Erin Byrne
dc.contributor.authorWade, Michael
dc.contributor.authorTong, Allison
dc.contributor.authorCotler, Max Joseph
dc.contributor.authorKuang, Jenevieve
dc.contributor.authorLugo, Alejandro Aponte
dc.contributor.authorZhang, Elizabeth Y.
dc.contributor.authorGraybiel, Ann M
dc.contributor.authorWhite, Forest M.
dc.contributor.authorLanger, Robert S
dc.contributor.authorCima, Michael J.
dc.date.accessioned2021-04-13T17:00:55Z
dc.date.available2021-04-13T17:00:55Z
dc.date.issued2020-09
dc.date.submitted2020-01
dc.identifier.issn2375-2548
dc.identifier.urihttps://hdl.handle.net/1721.1/130467
dc.description.abstractNeurochemical dysregulation underlies many pathologies and can be monitored by measuring the composition of brain interstitial fluid (ISF). Existing in vivo tools for sampling ISF do not enable measuring large rare molecules, such as proteins and neuropeptides, and thus cannot generate a complete picture of the neurochemical connectome. Our micro-invasive platform, composed of a nanofluidic pump coupled to a membrane-free probe, enables sampling multiple neural biomarkers in parallel. This platform outperforms the state of the art in low-flow pumps by offering low volume control (single stroke volumes, <3 nl) and bidirectional fluid flow (<100 nl/min) with negligible dead volume (<30 nl) and has been validated in vitro, ex vivo, and in vivo in rodents. ISF samples (<1.5 μL) can be processed via liquid chromatography-tandem mass spectrometry. These label-free liquid biopsies of the brain could yield a deeper understanding of the onset, mechanism, and progression of diverse neural pathologies.en_US
dc.description.sponsorshipNational Institute of Biomedical Imaging and Bioengineering (Grants R01 EB016101, R01 EB027717-01A1)en_US
dc.description.sponsorshipNSF (Grant 2016220817)en_US
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/sciadv.abb0657en_US
dc.rightsCreative Commons Attribution NonCommercial License 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.sourceScience Advancesen_US
dc.titlePlatform for micro-invasive membrane-free biochemical sampling of brain interstitial fluiden_US
dc.typeArticleen_US
dc.identifier.citationRaman, Ritu et al. "Platform for micro-invasive membrane-free biochemical sampling of brain interstitial fluid." Science Advances 6, 39 (September 2020): eabb0657 © 2020 American Association for the Advancement of Scienceen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentCenter for Precision Cancer Medicineen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.relation.journalScience Advancesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-03-19T15:11:12Z
dspace.orderedauthorsRaman, R; Rousseau, EB; Wade, M; Tong, A; Cotler, MJ; Kuang, J; Lugo, AA; Zhang, E; Graybiel, AM; White, FM; Langer, R; Cima, MJen_US
dspace.date.submission2021-03-19T15:11:13Z
mit.journal.volume6en_US
mit.journal.issue39en_US
mit.licensePUBLISHER_CC


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