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dc.contributor.authorBoopathy, Archana V
dc.contributor.authorMandal, Anasuya
dc.contributor.authorKulp, Daniel W.
dc.contributor.authorMenis, Sergey
dc.contributor.authorBennett, Nitasha R
dc.contributor.authorWatkins, Hannah C.
dc.contributor.authorWang, Wade
dc.contributor.authorMartin, Jacob T
dc.contributor.authorThai, Nikki Tessa
dc.contributor.authorHe, Yanpu
dc.contributor.authorSchief, William R.
dc.contributor.authorHammond, Paula T
dc.contributor.authorIrvine, Darrell J
dc.date.accessioned2021-04-27T21:26:03Z
dc.date.available2021-04-27T21:26:03Z
dc.date.issued2019-07
dc.date.submitted2019-02
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttps://hdl.handle.net/1721.1/130540
dc.description.abstractSustained exposure of lymphoid tissues to vaccine antigens promotes humoral immunity, but traditional bolus immunizations lead to rapid antigen clearance. We describe a technology to tailor vaccine kinetics in a needle-free platform translatable to human immunization. Solid pyramidal microneedle (MN) arrays were fabricated with silk fibroin protein tips encapsulating a stabilized HIV envelope trimer immunogen and adjuvant, supported on a dissolving polymer base. Upon brief skin application, vaccine-loaded silk tips are implanted in the epidermis/upper dermis where they release vaccine over a time period determined by the crystallinity of the silk matrix. Following MN immunization in mice, Env trimer was released over 2 wk in the skin, correlating with increased germinal center (GC) B cell responses, a ∼1,300-fold increase in serum IgG titers and a 16-fold increase in bone marrow (BM) plasma cells compared with bolus immunization. Thus, implantable MNs provide a practical means to substantially enhance humoral immunity to subunit vaccines.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (Awards UM1AI100663 and AI104715)en_US
dc.description.sponsorshipNational Cancer Institute (Grant P30-CA14051)en_US
dc.language.isoen
dc.publisherNational Academy of Sciencesen_US
dc.relation.isversionofhttp://dx.doi.org/10.1073/pnas.1902179116en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleEnhancing humoral immunity via sustained-release implantable microneedle patch vaccinationen_US
dc.typeArticleen_US
dc.identifier.citationBoopathy, Archana V. et al. "Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination." Proceedings of the National Academy of Sciences 116, 33 (July 2019): 16473-16478.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Soldier Nanotechnologiesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-08-20T18:27:48Z
dspace.date.submission2019-08-20T18:27:50Z
mit.journal.volume116en_US
mit.journal.issue33en_US
mit.metadata.statusComplete


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