Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Author(s)
Lee, J. Michelle; Kim, Chae Rin; Kim, Sion; Min, Junhong; Lee, Min-Ho; ... Show more
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Abstract
We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries.
Date issued
2021-09
URI
https://hdl.handle.net/1721.1/131288
Department
Massachusetts Institute of Technology. Department of Chemistry
Journal
Chemical Communications
Publisher
Royal Society of Chemistry (RSC)
Citation
Lee, J. Michelle et al. "Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor." Forthcoming in Chemical Communications. © 2021 The Royal Society of Chemistry
Version: Final published version
ISSN
1359-7345
1364-548X
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