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Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control
| dc.contributor.author | Xue, Yi | |
| dc.contributor.author | Rowlands, Christopher J. | |
| dc.contributor.author | So, Peter T. C. | |
| dc.date.accessioned | 2021-09-20T18:21:12Z | |
| dc.date.available | 2021-09-20T18:21:12Z | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/132159 | |
| dc.description.abstract | © 2015 SPIE. High resolution imaging in three dimension is important for biological research. RESOLFT (Reversible Saturable Optical Fluorescence Transitions) microscopy is one technique can achieve lateral super-resolution imaging. Twophoton microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. In this paper, we combine these two methods to realize three-dimensional high-resolution imaging. Spatial light modulator (SLM) is used as a flexible phase mask of the microscopy. Multiple super-resolution focuses as an array or in arbitrary positions could be generated by phase retrieval. This microscopy by SLM control could applied to parallel two-photon RESOLFT imaging or multiple spots tracking in high-resolution. | en_US |
| dc.publisher | SPIE-Intl Soc Optical Eng | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1117/12.2078509 | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.source | SPIE | en_US |
| dc.title | Parallel and flexible imaging using two-photon RESOLFT microscopy with spatial light modulator control | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Xue, Yi, Christopher J. Rowlands, and Peter T. C. So. “Parallel and Flexible Imaging Using Two-Photon RESOLFT Microscopy with Spatial Light Modulator Control.” Edited by Ammasi Periasamy, Peter T. C. So, and Karsten König. Multiphoton Microscopy in the Biomedical Sciences XV (March 5, 2015). doi:10.1117/12.2078509. | en_US |
| dc.eprint.version | Final published version | en_US |
| dc.type.uri | http://purl.org/eprint/type/ConferencePaper | en_US |
| eprint.status | http://purl.org/eprint/status/NonPeerReviewed | en_US |
| dc.date.updated | 2019-01-03T19:03:55Z | |
| dspace.orderedauthors | Xue, Yi; Rowlands, Christopher J.; So, Peter T. C. | en_US |
| dspace.embargo.terms | N | en_US |
| dspace.date.submission | 2019-04-04T14:45:45Z | |
| mit.license | PUBLISHER_POLICY | en_US |
| mit.metadata.status | Authority Work and Publication Information Needed |
