| dc.contributor.author | Lavin, Thomas K. | |
| dc.contributor.author | Jin, Lei | |
| dc.contributor.author | Wickersham, Ian R. | |
| dc.date.accessioned | 2021-10-12T16:01:51Z | |
| dc.date.available | 2021-10-12T16:01:51Z | |
| dc.date.issued | 2019-12 | |
| dc.date.submitted | 2019-07 | |
| dc.identifier.issn | 0891-0618 | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/132925 | |
| dc.description.abstract | Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors. | en_US |
| dc.description.sponsorship | National Institute of Mental Health (Awards U01MH106018, U01MH114829, and U19MH114830) | en_US |
| dc.publisher | Elsevier BV | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1016/j.jchemneu.2019.101661 | en_US |
| dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs License | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
| dc.source | Ian Wickersham | en_US |
| dc.title | Monosynaptic tracing: a step-by-step protocol | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Lavin, Thomas K. et al. "Monosynaptic tracing: a step-by-step protocol." Journal of Chemical Neuroanatomy 102 (December 2019): 101661. © 2019 Elsevier B.V. | en_US |
| dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
| dc.relation.journal | Journal of Chemical Neuroanatomy | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.date.submission | 2019-08-14T19:49:21Z | |
| mit.journal.volume | 102 | en_US |
| mit.metadata.status | Complete | en_US |
