Notice

This is not the latest version of this item. The latest version can be found at:https://dspace.mit.edu/handle/1721.1/133086.2

Show simple item record

dc.contributor.authorSteinbach, Sabine
dc.contributor.authorJalili-Firoozinezhad, Sasan
dc.contributor.authorSrinivasan, Sreenidhi
dc.contributor.authorMelo, Mariane B
dc.contributor.authorMiddleton, Sonya
dc.contributor.authorKonold, Timm
dc.contributor.authorCoad, Michael
dc.contributor.authorHammond, Paula T
dc.contributor.authorIrvine, Darrell J
dc.contributor.authorVordermeier, Martin
dc.contributor.authorKapur, Vivek
dc.date.accessioned2021-10-25T15:58:00Z
dc.date.available2021-10-25T15:58:00Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/133086
dc.description.abstract<jats:title>Abstract</jats:title><jats:p>Bovine tuberculosis (bTB) is a disease of livestock with severe and worldwide economic, animal welfare and zoonotic consequences. Application of test-and-slaughter-based control polices reliant on tuberculin skin testing has been the mainstay of bTB control in cattle. However, little is known about the temporal development of the bovine tuberculin skin test response at the dermal sites of antigen injection. To fill this knowledge gap, we applied minimally-invasive sampling microneedles (SMNs) for intradermal sampling of interstitial fluid at the tuberculin skin test sites in <jats:italic>Mycobacterium bovis</jats:italic> BCG-vaccinated calves and determined the temporal dynamics of a panel of 15 cytokines and chemokines in situ and in the peripheral blood. The results reveal an orchestrated and coordinated cytokine and local chemokine response, identified IL-1RA as a potential soluble biomarker of a positive tuberculin skin response, and confirmed the utility of IFN-γ and IP-10 for bTB detection in blood-based assays. Together, the results highlight the utility of SMNs to identify novel biomarkers and provide mechanistic insights on the intradermal cytokine and chemokine responses associated with the tuberculin skin test in BCG-sensitized cattle.</jats:p>en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionof10.1038/s41598-021-86398-6en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceScientific Reportsen_US
dc.titleTemporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedlesen_US
dc.typeArticleen_US
dc.identifier.citationSteinbach, Sabine, Jalili-Firoozinezhad, Sasan, Srinivasan, Sreenidhi, Melo, Mariane B, Middleton, Sonya et al. 2021. "Temporal dynamics of intradermal cytokine response to tuberculin in Mycobacterium bovis BCG-vaccinated cattle using sampling microneedles." Scientific Reports, 11 (1).
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-06-10T15:54:03Z
dspace.orderedauthorsSteinbach, S; Jalili-Firoozinezhad, S; Srinivasan, S; Melo, MB; Middleton, S; Konold, T; Coad, M; Hammond, PT; Irvine, DJ; Vordermeier, M; Kapur, Ven_US
dspace.date.submission2021-06-10T15:54:05Z
mit.journal.volume11en_US
mit.journal.issue1en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US
mit.metadata.statusAuthority Work and Publication Information Needed


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

VersionItemDateSummary

*Selected version