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dc.contributor.authorHong, Moo Sun
dc.contributor.authorKaur, Kawaljit
dc.contributor.authorSawant, Nishant
dc.contributor.authorJoshi, Sangeeta B
dc.contributor.authorVolkin, David B
dc.contributor.authorBraatz, Richard D
dc.date.accessioned2021-10-27T19:52:34Z
dc.date.available2021-10-27T19:52:34Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/133391
dc.description.abstractNonreplicating rotavirus vaccine (NRRV) candidates are being developed with the aim of serving the needs of developing countries. A significant proportion of the cost of manufacturing such vaccines is the purification in multiple chromatography steps. Crystallization has the potential to reduce purification costs and provide new product storage modality, improved operational flexibility, and reduced facility footprints. This communication describes a systematic approach for the design of the crystallization of an NRRV candidate, VP8 subunit proteins fused to the P2 epitope of tetanus toxin, using first-principles models and preliminary experimental data. The first-principles models are applied to literature data to obtain feasible crystallization conditions and lower bounds for nucleation and growth rates. Crystallization is then performed in a hanging-drop vapor diffusion system, resulting in the nucleation and growth of NRRV crystals. The crystals obtained in a scaled-up evaporative crystallization contain proteins truncated in the P2 region, but have no significant differences with the original samples in terms of antibody binding and overall conformational stability. These results demonstrate the promise of evaporative crystallization of the NRRV.
dc.language.isoen
dc.publisherWiley
dc.relation.isversionof10.1002/bit.27699
dc.rightsCreative Commons Attribution 4.0 International license
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceWiley
dc.titleCrystallization of a nonreplicating rotavirus vaccine candidate
dc.typeArticle
dc.relation.journalBiotechnology and Bioengineering
dc.eprint.versionFinal published version
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2021-06-09T13:53:49Z
dspace.orderedauthorsHong, MS; Kaur, K; Sawant, N; Joshi, SB; Volkin, DB; Braatz, RD
dspace.date.submission2021-06-09T13:53:51Z
mit.journal.volume118
mit.journal.issue4
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Needed


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