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dc.contributor.authorChao, Christy
dc.contributor.authorEngelward, Bevin P
dc.date.accessioned2021-12-07T18:01:50Z
dc.date.available2021-10-27T19:53:11Z
dc.date.available2021-12-07T18:01:50Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/1721.1/133500.2
dc.description.abstractCopyright © 2020 American Chemical Society. Environmental exposures have long been known to impact public health and safety. For example, exposures to airborne particulates, heavy metals in water, or certain industrial chemicals can contribute to aging and to risk of developing cancer and other diseases. Environmental factors can impact health in a variety of ways, but a key concern is DNA damage, which can lead to mutations that cause cancer. Cancer can take years to develop following chemical exposure; however, one way to predict carcinogenicity in a more practical time frame is by studying the chemical's ability to induce DNA damage. The comet assay (or single-cell gel electrophoresis assay) has been used successfully for genotoxicity testing. The comet assay allows for the detection of DNA strand breaks via analysis of DNA migration during electrophoresis. Previously, the Engelward laboratory, in collaboration with the Bhatia laboratory, developed the CometChip for measurements of DNA damage and repair. The CometChip is a high-throughput comet assay that improves user reproducibility and significantly shortens total assay time. Here, we describe how the high-throughput CometChip platform can be used to measure DNA damage in established cell lines, animal models, and human samples. We also discuss technical challenges associated with these studies and provide recommendations on how to achieve optimal results for researchers interested in adopting this assay.en_US
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionof10.1021/ACS.CHEMRESTOX.9B00393en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleApplications of CometChip for Environmental Health Studiesen_US
dc.typeArticleen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.relation.journalChemical Research in Toxicologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-08-27T16:49:29Z
dspace.orderedauthorsChao, C; Engelward, BPen_US
dspace.date.submission2021-08-27T16:49:31Z
mit.journal.volume33en_US
mit.journal.issue7en_US
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusPublication Information Neededen_US


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