dc.contributor.author | Michelow, Ian C | |
dc.contributor.author | Park, Sangshin | |
dc.contributor.author | Tsai, Shu-Whei | |
dc.contributor.author | Rayta, Bonnie | |
dc.contributor.author | Pasaje, Charisse Flerida A | |
dc.contributor.author | Nelson, Sara | |
dc.contributor.author | Early, Angela M | |
dc.contributor.author | Frosch, Anne P | |
dc.contributor.author | Ayodo, George | |
dc.contributor.author | Raj, Dipak K | |
dc.contributor.author | Nixon, Christina E | |
dc.contributor.author | Nixon, Christian P | |
dc.contributor.author | Pond-Tor, Sunthorn | |
dc.contributor.author | Friedman, Jennifer F | |
dc.contributor.author | Fried, Michal | |
dc.contributor.author | Duffy, Patrick E | |
dc.contributor.author | Le Roch, Karine G | |
dc.contributor.author | Niles, Jacquin C | |
dc.contributor.author | Kurtis, Jonathan D | |
dc.date.accessioned | 2021-10-27T19:53:40Z | |
dc.date.available | 2021-10-27T19:53:40Z | |
dc.date.issued | 2021-09-06 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/133588 | |
dc.description.abstract | <jats:p>We previously identified a Plasmodium falciparum (Pf) protein of unknown function encoded by a single-copy gene, PF3D7_1134300, as a target of antibodies in plasma of Tanzanian children in a whole-proteome differential screen. Here we characterize this protein as a blood-stage antigen that localizes to the surface membranes of both parasitized erythrocytes and merozoites, hence its designation as Pf erythrocyte membrane and merozoite antigen 1 (PfEMMA1). Mouse anti-PfEMMA1 antisera and affinity-purified human anti-PfEMMA1 antibodies inhibited growth of P. falciparum strains by up to 68% in growth inhibition assays. Following challenge with uniformly fatal Plasmodium berghei (Pb) ANKA, up to 40% of mice immunized with recombinant PbEMMA1 self-cured, and median survival of lethally infected mice was up to 2.6-fold longer than controls (21 vs. 8 d, P = 0.005). Furthermore, high levels of naturally acquired human anti-PfEMMA1 antibodies were associated with a 46% decrease in parasitemia over 2.5 yr of follow-up of Tanzanian children. Together, these findings suggest that antibodies to PfEMMA1 mediate protection against malaria.</jats:p> | en_US |
dc.language.iso | en | |
dc.publisher | Rockefeller University Press | en_US |
dc.relation.isversionof | 10.1084/jem.20200170 | en_US |
dc.rights | Creative Commons Attribution 4.0 International license | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Rockefeller University Press | en_US |
dc.title | A newly characterized malaria antigen on erythrocyte and merozoite surfaces induces parasite inhibitory antibodies | en_US |
dc.type | Article | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | |
dc.relation.journal | Journal of Experimental Medicine | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2021-09-10T13:32:45Z | |
dspace.orderedauthors | Michelow, IC; Park, S; Tsai, S-W; Rayta, B; Pasaje, CFA; Nelson, S; Early, AM; Frosch, AP; Ayodo, G; Raj, DK; Nixon, CE; Nixon, CP; Pond-Tor, S; Friedman, JF; Fried, M; Duffy, PE; Le Roch, KG; Niles, JC; Kurtis, JD | en_US |
dspace.date.submission | 2021-09-10T13:32:48Z | |
mit.journal.volume | 218 | en_US |
mit.journal.issue | 9 | en_US |
mit.license | PUBLISHER_CC | |
mit.metadata.status | Authority Work and Publication Information Needed | en_US |