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dc.contributor.authorChoi, Jonghoon
dc.contributor.authorRoutenberg Love, Kerry
dc.contributor.authorGong, Yuan
dc.contributor.authorGierahn, Todd M
dc.contributor.authorLove, J Christopher
dc.date.accessioned2021-10-27T19:57:15Z
dc.date.available2021-10-27T19:57:15Z
dc.date.issued2011
dc.identifier.urihttps://hdl.handle.net/1721.1/133929
dc.description.abstractWe present here a new method to enhance the detection of secreted cytokines and chemokines from single human mononuclear cells. The technique uses a hybridization chain reaction (HCR) to amplify signals resulting from sandwich immunoassays. This immuno-HCR employs oligonucleotide-based initiators covalently linked to antibodies to propagate a chain reaction of hybridization events involving a pair of complementary hairpin oligomers bearing fluorescent labels. Integrating this strategy for signal amplification with microengraving (a soft lithographic method for printing arrays of secreted proteins from thousands of single cells) improves both the limits of detection and sensitivity for cytokines and chemokines captured from individual cells by an average of 200-fold relative to methods for direct detection by fluoresence. This approach should enhance the utility of microengraving for defining the immunological signatures of diseases and responses to interventional therapies based on multiplexed single-cell analysis. © 2011 American Chemical Society.
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)
dc.relation.isversionof10.1021/ac2013916
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.sourcePMC
dc.titleImmuno-Hybridization Chain Reaction for Enhancing Detection of Individual Cytokine-Secreting Human Peripheral Mononuclear Cells
dc.typeArticle
dc.identifier.citationChoi, Jonghoon, et al. "Immuno-Hybridization Chain Reaction for Enhancing Detection of Individual Cytokine-Secreting Human Peripheral Mononuclear Cells." Analytical Chemistry 83 17 (2011): 6890-95.
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineering
dc.contributor.departmentRagon Institute of MGH, MIT and Harvard
dc.relation.journalAnalytical Chemistry
dc.eprint.versionAuthor's final manuscript
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2019-09-09T17:24:45Z
dspace.orderedauthorsChoi, J; Routenberg Love, K; Gong, Y; Gierahn, TM; Love, JC
dspace.date.submission2019-09-09T17:24:47Z
mit.journal.volume83
mit.journal.issue17
mit.metadata.statusAuthority Work and Publication Information Needed


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