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dc.contributor.authorYildirim, Murat
dc.contributor.authorHu, Ming
dc.contributor.authorLe, Nhat M
dc.contributor.authorSugihara, Hiroki
dc.contributor.authorSo, Peter TC
dc.contributor.authorSur, Mriganka
dc.date.accessioned2021-10-27T19:58:17Z
dc.date.available2021-10-27T19:58:17Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/1721.1/134136
dc.description.abstract© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. The structure of brain regions is assumed to correlate with their function, but there are very few instances in which the relationship has been demonstrated in the live brain. This is due to the difficulty of simultaneously measuring functional and structural properties of brain areas, particularly at cellular resolution. Here, we performed label-free, third-harmonic generation (THG) microscopy to obtain a key structural signature of cortical areas, their effective attenuation lengths (EAL), in the vertical columns of functionally defined primary visual cortex and five adjacent visual areas in awake mice. EALs measured by THG microscopy in the cortex and white matter showed remarkable correspondence with the functional retinotopic sign map of each area. Structural features such as cytoarchitecture, myeloarchitecture and blood vessel architecture were correlated with areal EAL values, suggesting that EAL is a function of these structural features as an optical property of these areas. These results demonstrate for the first time a strong relationship between structural substrates of visual cortical areas and their functional representation maps in vivo. This study may also help in understanding the coupling between structure and function in other animal models as well as in humans.
dc.language.isoen
dc.publisherThe Optical Society
dc.relation.isversionof10.1364/BOE.396962
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.sourceOSA Publishing
dc.titleQuantitative third-harmonic generation imaging of mouse visual cortex areas reveals correlations between functional maps and structural substrates
dc.typeArticle
dc.relation.journalBiomedical Optics Express
dc.eprint.versionFinal published version
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2021-03-19T14:38:28Z
dspace.orderedauthorsYildirim, M; Hu, M; Le, NM; Sugihara, H; So, PTC; Sur, M
dspace.date.submission2021-03-19T14:38:32Z
mit.journal.volume11
mit.journal.issue10
mit.licensePUBLISHER_POLICY
mit.metadata.statusAuthority Work and Publication Information Needed


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