Heat activates the AAA+ HslUV protease by melting an axial autoinhibitory plug

Author(s)
Baytshtok, Vladimir; Fei, Xue; Shih, Tsai-Ting; Grant, Robert A; Santos, Justin C; ... Show more
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Abstract
© 2020 The Author(s) At low temperatures, protein degradation by the AAA+ HslUV protease is very slow. New crystal structures reveal that residues in the intermediate domain of the HslU6 unfoldase can plug its axial channel, blocking productive substrate binding and subsequent unfolding, translocation, and degradation by the HslV12 peptidase. Biochemical experiments with wild-type and mutant enzymes support a model in which heat-induced melting of this autoinhibitory plug activates HslUV proteolysis.
Date issued
2021
URI
https://hdl.handle.net/1721.1/134390
Department
Massachusetts Institute of Technology. Department of Biology
Journal
Cell Reports
Publisher
Elsevier BV
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