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dc.contributor.authorThomas, Sydney P
dc.contributor.authorHoang, Trish T
dc.contributor.authorRessler, Valerie T
dc.contributor.authorRaines, Ronald T
dc.date.accessioned2021-10-27T20:10:24Z
dc.date.available2021-10-27T20:10:24Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/1721.1/135030
dc.description.abstract© 2018 Steiman-Shimony et al. Angiogenin (ANG) is a secretory ribonuclease that promotes the proliferation of endothelial cells, leading to angiogenesis. This function relies on its ribonucleolytic activity, which is low for simple RNA substrates. Upon entry into the cytosol, ANG is sequestered by the ribonuclease inhibitor protein (RNH1). We find that ANG is a potent cytotoxin for RNH1-knockout HeLa cells, belying its inefficiency as a nonspecific catalyst. The toxicity does, however, rely on the ribonucleolytic activity of ANG and a cytosolic localization, which lead to the accumulation of particular tRNA fragments (tRFs), such as tRF-5 Gly-GCC. These up-regulated tRFs are highly cytotoxic at physiological concentrations. Although ANG is well-known for its promotion of cell growth, our results reveal that ANG can also cause cell death.
dc.language.isoen
dc.publisherCold Spring Harbor Laboratory
dc.relation.isversionof10.1261/RNA.065516.117
dc.rightsCreative Commons Attribution NonCommercial License 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceGenes and Development
dc.titleHuman angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor
dc.typeArticle
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistry
dc.relation.journalRNA
dc.eprint.versionFinal published version
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2019-09-16T14:50:25Z
dspace.orderedauthorsThomas, SP; Hoang, TT; Ressler, VT; Raines, RT
dspace.date.submission2019-09-16T14:50:27Z
mit.journal.volume24
mit.journal.issue8
mit.metadata.statusAuthority Work and Publication Information Needed


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