Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA
Author(s)
Bhattacharyya, Roby P; Walker, Mark; Boykin, Rich; Son, Sophie S; Liu, Jamin; Hachey, Austin C; Ma, Peijun; Wu, Lidan; Choi, Kyungyong; Cummins, Kaelyn C; Benson, Maura; Skerry, Jennifer; Ryu, Hyunryul; Wong, Sharon Y; Goldberg, Marcia B; Han, Jongyoon; Pierce, Virginia M; Cosimi, Lisa A; Shoresh, Noam; Livny, Jonathan; Beechem, Joseph; Hung, Deborah T; ... Show more Show less
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© 2019, The Author(s). Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified.
Date issued
2019Department
Massachusetts Institute of Technology. Research Laboratory of Electronics; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Biological EngineeringJournal
Scientific Reports
Publisher
Springer Science and Business Media LLC