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dc.contributor.authorOdermatt, Pascal D
dc.contributor.authorMiettinen, Teemu P
dc.contributor.authorLemière, Joël
dc.contributor.authorKang, Joon Ho
dc.contributor.authorBostan, Emrah
dc.contributor.authorManalis, Scott R
dc.contributor.authorHuang, Kerwyn Casey
dc.contributor.authorChang, Fred
dc.date.accessioned2021-10-27T20:24:21Z
dc.date.available2021-10-27T20:24:21Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/135632
dc.description.abstract<jats:p>Intracellular density impacts the physical nature of the cytoplasm and can globally affect cellular processes, yet density regulation remains poorly understood. Here, using a new quantitative phase imaging method, we determined that dry-mass density in fission yeast is maintained in a narrow distribution and exhibits homeostatic behavior. However, density varied during the cell cycle, decreasing during G2, increasing in mitosis and cytokinesis, and dropping rapidly at cell birth. These density variations were explained by a constant rate of biomass synthesis, coupled to slowdown of volume growth during cell division and rapid expansion post-cytokinesis. Arrest at specific cell-cycle stages exacerbated density changes. Spatially heterogeneous patterns of density suggested links between density regulation, tip growth, and intracellular osmotic pressure. Our results demonstrate that systematic density variations during the cell cycle are predominantly due to modulation of volume expansion, and reveal functional consequences of density gradients and cell-cycle arrests.</jats:p>
dc.language.isoen
dc.publishereLife Sciences Publications, Ltd
dc.relation.isversionof10.7554/elife.64901
dc.rightsCreative Commons Attribution 4.0 International license
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceeLife
dc.titleVariations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast
dc.typeArticle
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MIT
dc.contributor.departmentMassachusetts Institute of Technology. Department of Physics
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineering
dc.relation.journaleLife
dc.eprint.versionFinal published version
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2021-09-07T18:01:01Z
dspace.orderedauthorsOdermatt, PD; Miettinen, TP; Lemière, J; Kang, JH; Bostan, E; Manalis, SR; Huang, KC; Chang, F
dspace.date.submission2021-09-07T18:01:02Z
mit.journal.volume10
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Needed


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