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dc.contributor.authorGuan, Zhuo
dc.contributor.authorQuiñones-Frías, Monica C
dc.contributor.authorAkbergenova, Yulia
dc.contributor.authorLittleton, J Troy
dc.date.accessioned2021-10-27T20:30:19Z
dc.date.available2021-10-27T20:30:19Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/1721.1/136003
dc.description.abstract© 2020, eLife Sciences Publications Ltd. All rights reserved. Synchronous neurotransmitter release is triggered by Ca2+ binding to the synaptic vesicle protein Synaptotagmin 1, while asynchronous fusion and short-term facilitation is hypothesized to be mediated by plasma membrane-localized Synaptotagmin 7 (SYT7). We generated mutations in Drosophila Syt7 to determine if it plays a conserved role as the Ca2+ sensor for these processes. Electrophysiology and quantal imaging revealed evoked release was elevated 2-fold. Syt7 mutants also had a larger pool of readily-releasable vesicles, faster recovery following stimulation, and intact facilitation. Syt1/Syt7 double mutants displayed more release than Syt1 mutants alone, indicating SYT7 does not mediate the residual asynchronous release remaining in the absence of SYT1. SYT7 localizes to an internal membrane tubular network within the peri-active zone, but does not enrich at active zones. These findings indicate the two Ca2+ sensor model of SYT1 and SYT7 mediating all phases of neurotransmitter release and facilitation is not applicable at Drosophila synapses.
dc.language.isoen
dc.publishereLife Sciences Publications, Ltd
dc.relation.isversionof10.7554/ELIFE.55443
dc.rightsCreative Commons Attribution 4.0 International license
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceeLife
dc.titleDrosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner
dc.typeArticle
dc.relation.journaleLife
dc.eprint.versionFinal published version
dc.type.urihttp://purl.org/eprint/type/JournalArticle
eprint.statushttp://purl.org/eprint/status/PeerReviewed
dc.date.updated2021-07-21T15:42:13Z
dspace.orderedauthorsGuan, Z; Quiñones-Frías, MC; Akbergenova, Y; Littleton, JT
dspace.date.submission2021-07-21T15:42:15Z
mit.journal.volume9
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Needed


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