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Intravital imaging of mouse embryos

dc.contributor.authorHuang, Qiang
dc.contributor.authorCohen, Malkiel A.
dc.contributor.authorAlsina, Fernando C.
dc.contributor.authorDevlin, Garth
dc.contributor.authorGarrett, Aliesha
dc.contributor.authorMcKey, Jennifer
dc.contributor.authorHavlik, Patrick
dc.contributor.authorRakhilin, Nikolai
dc.contributor.authorWang, Ergang
dc.contributor.authorXiang, Kun
dc.contributor.authorMathews, Parker
dc.contributor.authorWang, Lihua
dc.contributor.authorBock, Cheryl
dc.contributor.authorRuthig, Victor
dc.contributor.authorWang, Yi
dc.contributor.authorNegrete, Marcos
dc.contributor.authorWong, Chi Wut
dc.contributor.authorMurthy, Preetish K. L.
dc.contributor.authorZhang, Shupei
dc.contributor.authorDaniel, Andrea R.
dc.contributor.authorKirsch, David G.
dc.contributor.authorKang, Yubin
dc.contributor.authorCapel, Blanche
dc.contributor.authorAsokan, Aravind
dc.contributor.authorSilver, Debra L.
dc.contributor.authorJaenisch, Rudolf
dc.contributor.authorShen, Xiling
dc.date.accessioned2022-03-14T17:43:01Z
dc.date.available2021-10-27T20:36:28Z
dc.date.available2022-03-14T17:43:01Z
dc.date.issued2020-04
dc.date.submitted2019-10
dc.identifier.issn0036-8075
dc.identifier.issn1095-9203
dc.identifier.urihttps://hdl.handle.net/1721.1/136659.2
dc.description.abstract© 2020 The Authors. Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.en_US
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/science.aba0210en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleIntravital imaging of mouse embryosen_US
dc.typeArticleen_US
dc.contributor.departmentWhitehead Institute for Biomedical Research
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biology
dc.relation.journalScienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-07-19T17:36:41Z
dspace.orderedauthorsHuang, Q; Cohen, MA; Alsina, FC; Devlin, G; Garrett, A; McKey, J; Havlik, P; Rakhilin, N; Wang, E; Xiang, K; Mathews, P; Wang, L; Bock, C; Ruthig, V; Wang, Y; Negrete, M; Wong, CW; Murthy, PKL; Zhang, S; Daniel, AR; Kirsch, DG; Kang, Y; Capel, B; Asokan, A; Silver, DL; Jaenisch, R; Shen, Xen_US
dspace.date.submission2021-07-19T17:36:44Z
mit.journal.volume368en_US
mit.journal.issue6487en_US
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusAuthority Work Neededen_US


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21721.1/136659.2*2022-03-14T17:40:57ZPublication information verified/added.
11721.1/1366592021-10-27T20:36:28Z

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