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A genetically encoded near-infrared fluorescent calcium ion indicator

dc.contributor.authorQian, Yong
dc.contributor.authorPiatkevich, Kiryl D.
dc.contributor.authorMc Larney, Benedict
dc.contributor.authorAbdelfattah, Ahmed S.
dc.contributor.authorMehta, Sohum
dc.contributor.authorMurdock, Mitchell H.
dc.contributor.authorGottschalk, Sven
dc.contributor.authorMolina, Rosana S.
dc.contributor.authorZhang, Wei
dc.contributor.authorChen, Yingche
dc.contributor.authorWu, Jiahui
dc.contributor.authorDrobizhev, Mikhail
dc.contributor.authorHughes, Thomas E.
dc.contributor.authorZhang, Jin
dc.contributor.authorSchreiter, Eric R.
dc.contributor.authorShoham, Shy
dc.contributor.authorRazansky, Daniel
dc.contributor.authorBoyden, Edward S.
dc.contributor.authorCampbell, Robert E.
dc.date.accessioned2022-06-13T14:56:13Z
dc.date.available2021-11-19T16:19:10Z
dc.date.available2022-06-13T14:56:13Z
dc.date.issued2019-01
dc.date.submitted2018-05
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttps://hdl.handle.net/1721.1/138166.2
dc.description.abstract© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41592-018-0294-6en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleA genetically encoded near-infrared fluorescent calcium ion indicatoren_US
dc.typeArticleen_US
dc.identifier.citationQian, Yong, Piatkevich, Kiryl D, Mc Larney, Benedict, Abdelfattah, Ahmed S, Mehta, Sohum et al. 2019. "A genetically encoded near-infrared fluorescent calcium ion indicator." Nature Methods, 16 (2).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Media Laboratory
dc.contributor.departmentMcGovern Institute for Brain Research at MIT
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-11-19T16:15:40Z
dspace.orderedauthorsQian, Y; Piatkevich, KD; Mc Larney, B; Abdelfattah, AS; Mehta, S; Murdock, MH; Gottschalk, S; Molina, RS; Zhang, W; Chen, Y; Wu, J; Drobizhev, M; Hughes, TE; Zhang, J; Schreiter, ER; Shoham, S; Razansky, D; Boyden, ES; Campbell, REen_US
dspace.date.submission2021-11-19T16:15:42Z
mit.journal.volume16en_US
mit.journal.issue2en_US
mit.licensePUBLISHER_POLICY
mit.metadata.statusAuthority Work Neededen_US


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