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A genetically encoded near-infrared fluorescent calcium ion indicator

dc.contributor.authorQian, Yong
dc.contributor.authorPiatkevich, Kiryl D
dc.contributor.authorMc Larney, Benedict
dc.contributor.authorAbdelfattah, Ahmed S
dc.contributor.authorMehta, Sohum
dc.contributor.authorMurdock, Mitchell H
dc.contributor.authorGottschalk, Sven
dc.contributor.authorMolina, Rosana S
dc.contributor.authorZhang, Wei
dc.contributor.authorChen, Yingche
dc.contributor.authorWu, Jiahui
dc.contributor.authorDrobizhev, Mikhail
dc.contributor.authorHughes, Thomas E
dc.contributor.authorZhang, Jin
dc.contributor.authorSchreiter, Eric R
dc.contributor.authorShoham, Shy
dc.contributor.authorRazansky, Daniel
dc.contributor.authorBoyden, Edward S
dc.contributor.authorCampbell, Robert E
dc.date.accessioned2021-11-19T16:19:10Z
dc.date.available2021-11-19T16:19:10Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/1721.1/138166
dc.description.abstract© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.en_US
dc.language.isoen
dc.publisherSpringer Natureen_US
dc.relation.isversionof10.1038/S41592-018-0294-6en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleA genetically encoded near-infrared fluorescent calcium ion indicatoren_US
dc.typeArticleen_US
dc.identifier.citationQian, Yong, Piatkevich, Kiryl D, Mc Larney, Benedict, Abdelfattah, Ahmed S, Mehta, Sohum et al. 2019. "A genetically encoded near-infrared fluorescent calcium ion indicator." Nature Methods, 16 (2).
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2021-11-19T16:15:40Z
dspace.orderedauthorsQian, Y; Piatkevich, KD; Mc Larney, B; Abdelfattah, AS; Mehta, S; Murdock, MH; Gottschalk, S; Molina, RS; Zhang, W; Chen, Y; Wu, J; Drobizhev, M; Hughes, TE; Zhang, J; Schreiter, ER; Shoham, S; Razansky, D; Boyden, ES; Campbell, REen_US
dspace.date.submission2021-11-19T16:15:42Z
mit.journal.volume16en_US
mit.journal.issue2en_US
mit.licensePUBLISHER_POLICY
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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21721.1/138166.22022-06-13T14:54:23ZPublication information verified/added.
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