Highly Parallel Profiling of Cas9 Variant Specificity

Author(s)
Schmid-Burgk, JL; Gao, L; Li, D; Gardner, Z; Strecker, J; ... Show more
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Abstract
© 2020 Elsevier Inc. Schmid-Burgk et al. develop tagmentation-based tag integration site sequencing (TTISS), a rapid, streamlined protocol for analyzing double-strand breaks such as those created by CRISPR nucleases. Using TTISS, they comprehensively assess Cas9 variants, revealing a trade-off between specificity and activity and identifying LZ3 Cas9, a variant with a unique +1 insertion profile.
Date issued
2020-05-21
URI
https://hdl.handle.net/1721.1/138391
Department
McGovern Institute for Brain Research at MIT; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Department of Biological Engineering; Howard Hughes Medical Institute
Journal
Molecular Cell
Publisher
Elsevier BV
Citation
Schmid-Burgk, JL, Gao, L, Li, D, Gardner, Z, Strecker, J et al. 2020. "Highly Parallel Profiling of Cas9 Variant Specificity." Molecular Cell, 78 (4).
Version: Author's final manuscript
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