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dc.contributor.authorZeming, Kerwin Kwek
dc.contributor.authorLu, Ri
dc.contributor.authorWoo, Kai Lee
dc.contributor.authorSun, Guoyun
dc.contributor.authorQuek, Kai Yun
dc.contributor.authorCheow, Lih Feng
dc.contributor.authorChen, Chia-Hung
dc.contributor.authorHan, Jongyoon
dc.contributor.authorLim, Shir Lynn
dc.date.accessioned2022-06-30T17:14:21Z
dc.date.available2022-06-30T17:14:21Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/143614
dc.description.abstract© 2021 American Chemical Society. Enzymatic secretion of immune cells (leukocytes) plays a dominant role in host immune responses to a myriad of biological triggers, including infections, cancers, and cardiovascular diseases. Current tools to probe these leukocytes inadequately profile these vital biomarkers; the need for sample preprocessing steps of cell lysis, labeling, washing, and pipetting inevitably triggers the cells, changes its basal state, and dilutes the individual cell secretion in bulk assays. Using a fully integrated system for multiplexed profiling of native immune single-cell enzyme secretion from 50 μL of undiluted blood, we eliminate sample handling. With a total analysis time of 60 min, the integrated platform performs six tasks of leukocyte extraction, cell washing, fluorescent enzyme substrate mixing, single-cell droplet making, droplet incubation, and real-time readout for leukocyte secretion profiling of neutrophil elastase, granzyme B, and metalloproteinase. We calibrated the device, optimized the protocols, and tested the leukocyte secretion of acute heart failure (AHF) patients at admission and predischarge. This paper highlights the presence of single-cell enzymatic immune phenotypes independent of CD marker labeling, which could potentially elucidate the innate immune response states. We found that patients recovering from AHF showed a corresponding reduction in immune-cell enzymatic secretion levels and donor-specific enzymatic signatures were observed, which suggests patient-to-patient heterogeneous immune response. This platform presents opportunities to elucidate the complexities of the immune response from a single drop of blood and bridge the current technological, biological, and medical gap in understanding immune response and biological triggers.en_US
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionof10.1021/ACS.ANALCHEM.0C03512en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourceACSen_US
dc.titleMultiplexed Single-Cell Leukocyte Enzymatic Secretion Profiling from Whole Blood Reveals Patient-Specific Immune Signatureen_US
dc.typeArticleen_US
dc.identifier.citationZeming, Kerwin Kwek, Lu, Ri, Woo, Kai Lee, Sun, Guoyun, Quek, Kai Yun et al. 2021. "Multiplexed Single-Cell Leukocyte Enzymatic Secretion Profiling from Whole Blood Reveals Patient-Specific Immune Signature." Analytical Chemistry, 93 (10).
dc.contributor.departmentSingapore-MIT Alliance in Research and Technology (SMART)
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2022-06-30T16:33:23Z
dspace.orderedauthorsZeming, KK; Lu, R; Woo, KL; Sun, G; Quek, KY; Cheow, LF; Chen, C-H; Han, J; Lim, SLen_US
dspace.date.submission2022-06-30T16:33:29Z
mit.journal.volume93en_US
mit.journal.issue10en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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