dc.contributor.author | Schubert, Max G | |
dc.contributor.author | Goodman, Daniel B | |
dc.contributor.author | Wannier, Timothy M | |
dc.contributor.author | Kaur, Divjot | |
dc.contributor.author | Farzadfard, Fahim | |
dc.contributor.author | Lu, Timothy K | |
dc.contributor.author | Shipman, Seth L | |
dc.contributor.author | Church, George M | |
dc.date.accessioned | 2022-07-15T13:29:12Z | |
dc.date.available | 2022-07-15T13:29:12Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/143760 | |
dc.description.abstract | <jats:title>Significance</jats:title>
<jats:p>We report a methodology for the pooled construction of mutants bearing precise genomic sequence variations and multiplex phenotypic characterization of these mutants using next-generation sequencing (NGS). Unlike existing techniques depending on CRISPR-Cas–directed genomic breaks for genome editing, this strategy instead uses single-stranded DNA produced by a retron element for recombineering. This enables libraries of millions of elements to be constructed and offers relaxed design constraints which permit natural DNA or random variation to be used as inputs.</jats:p> | en_US |
dc.language.iso | en | |
dc.publisher | Proceedings of the National Academy of Sciences | en_US |
dc.relation.isversionof | 10.1073/PNAS.2018181118 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | PNAS | en_US |
dc.title | High-throughput functional variant screens via in vivo production of single-stranded DNA | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Schubert, Max G, Goodman, Daniel B, Wannier, Timothy M, Kaur, Divjot, Farzadfard, Fahim et al. 2021. "High-throughput functional variant screens via in vivo production of single-stranded DNA." Proceedings of the National Academy of Sciences of the United States of America, 118 (18). | |
dc.contributor.department | Massachusetts Institute of Technology. Research Laboratory of Electronics | |
dc.relation.journal | Proceedings of the National Academy of Sciences of the United States of America | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2022-07-15T13:08:41Z | |
dspace.orderedauthors | Schubert, MG; Goodman, DB; Wannier, TM; Kaur, D; Farzadfard, F; Lu, TK; Shipman, SL; Church, GM | en_US |
dspace.date.submission | 2022-07-15T13:08:42Z | |
mit.journal.volume | 118 | en_US |
mit.journal.issue | 18 | en_US |
mit.license | PUBLISHER_POLICY | |
mit.metadata.status | Authority Work and Publication Information Needed | en_US |