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dc.contributor.authorSchubert, Max G
dc.contributor.authorGoodman, Daniel B
dc.contributor.authorWannier, Timothy M
dc.contributor.authorKaur, Divjot
dc.contributor.authorFarzadfard, Fahim
dc.contributor.authorLu, Timothy K
dc.contributor.authorShipman, Seth L
dc.contributor.authorChurch, George M
dc.date.accessioned2022-07-15T13:29:12Z
dc.date.available2022-07-15T13:29:12Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/143760
dc.description.abstract<jats:title>Significance</jats:title> <jats:p>We report a methodology for the pooled construction of mutants bearing precise genomic sequence variations and multiplex phenotypic characterization of these mutants using next-generation sequencing (NGS). Unlike existing techniques depending on CRISPR-Cas–directed genomic breaks for genome editing, this strategy instead uses single-stranded DNA produced by a retron element for recombineering. This enables libraries of millions of elements to be constructed and offers relaxed design constraints which permit natural DNA or random variation to be used as inputs.</jats:p>en_US
dc.language.isoen
dc.publisherProceedings of the National Academy of Sciencesen_US
dc.relation.isversionof10.1073/PNAS.2018181118en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleHigh-throughput functional variant screens via in vivo production of single-stranded DNAen_US
dc.typeArticleen_US
dc.identifier.citationSchubert, Max G, Goodman, Daniel B, Wannier, Timothy M, Kaur, Divjot, Farzadfard, Fahim et al. 2021. "High-throughput functional variant screens via in vivo production of single-stranded DNA." Proceedings of the National Academy of Sciences of the United States of America, 118 (18).
dc.contributor.departmentMassachusetts Institute of Technology. Research Laboratory of Electronics
dc.relation.journalProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2022-07-15T13:08:41Z
dspace.orderedauthorsSchubert, MG; Goodman, DB; Wannier, TM; Kaur, D; Farzadfard, F; Lu, TK; Shipman, SL; Church, GMen_US
dspace.date.submission2022-07-15T13:08:42Z
mit.journal.volume118en_US
mit.journal.issue18en_US
mit.licensePUBLISHER_POLICY
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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