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dc.contributor.authorMu, Weihua
dc.contributor.authorKong, Jing
dc.contributor.authorCao, Jianshu
dc.date.accessioned2022-07-22T15:22:27Z
dc.date.available2022-07-22T15:22:27Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/1721.1/143971
dc.description.abstractThe cooperativity of a monomeric enzyme arises from dynamic correlation instead of spatial correlation and is a consequence of nonequilibrium conformation fluctuations. We investigate the conformation-modulated kinetics of human glucokinase, a monomeric enzyme with important physiological functions, using a five-state kinetic model. We derive the non-Michealis-Menten (MM) correction term of the activity (i.e., turnover rate), predict its relationship to cooperativity, and reveal the violation of conformational detailed balance. Most importantly, we reproduce and explain the observed resonance effect in human glucokinase (i.e., maximal cooperativity when the conformational fluctuation rate is comparable to the catalytic rate). With the realistic parameters, our theoretical results are in quantitative agreement with the reported measurement by Miller and co-workers. The analysis can be extended to a general chemical network beyond the five-state model, suggesting the generality of kinetic cooperativity and resonance.en_US
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionof10.1021/ACS.JPCLETT.1C00438en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceMIT web domainen_US
dc.titleUnderstanding the Optimal Cooperativity of Human Glucokinase: Kinetic Resonance in Nonequilibrium Conformational Fluctuationsen_US
dc.typeArticleen_US
dc.identifier.citationMu, Weihua, Kong, Jing and Cao, Jianshu. 2021. "Understanding the Optimal Cooperativity of Human Glucokinase: Kinetic Resonance in Nonequilibrium Conformational Fluctuations." Journal of Physical Chemistry Letters, 12 (11).
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistry
dc.relation.journalJournal of Physical Chemistry Lettersen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2022-07-22T15:19:08Z
dspace.orderedauthorsMu, W; Kong, J; Cao, Jen_US
dspace.date.submission2022-07-22T15:19:09Z
mit.journal.volume12en_US
mit.journal.issue11en_US
mit.licenseOPEN_ACCESS_POLICY
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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