| dc.contributor.author | Kannan, Soumya | |
| dc.contributor.author | Altae-Tran, Han | |
| dc.contributor.author | Jin, Xin | |
| dc.contributor.author | Madigan, Victoria J | |
| dc.contributor.author | Oshiro, Rachel | |
| dc.contributor.author | Makarova, Kira S | |
| dc.contributor.author | Koonin, Eugene V | |
| dc.contributor.author | Zhang, Feng | |
| dc.date.accessioned | 2022-08-22T16:54:47Z | |
| dc.date.available | 2022-08-22T16:54:47Z | |
| dc.date.issued | 2022-02 | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/144412 | |
| dc.description.abstract | CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus. | en_US |
| dc.language.iso | en | |
| dc.publisher | Springer Science and Business Media LLC | en_US |
| dc.relation.isversionof | 10.1038/s41587-021-01030-2 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | Compact RNA editors with small Cas13 proteins | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Kannan, Soumya, Altae-Tran, Han, Jin, Xin, Madigan, Victoria J, Oshiro, Rachel et al. 2022. "Compact RNA editors with small Cas13 proteins." Nature Biotechnology, 40 (2). | |
| dc.contributor.department | Howard Hughes Medical Institute | |
| dc.contributor.department | McGovern Institute for Brain Research at MIT | |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | |
| dc.relation.journal | Nature Biotechnology | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dc.date.updated | 2022-08-22T16:43:45Z | |
| dspace.orderedauthors | Kannan, S; Altae-Tran, H; Jin, X; Madigan, VJ; Oshiro, R; Makarova, KS; Koonin, EV; Zhang, F | en_US |
| dspace.date.submission | 2022-08-22T16:43:46Z | |
| mit.journal.volume | 40 | en_US |
| mit.journal.issue | 2 | en_US |
| mit.license | OPEN_ACCESS_POLICY | |
| mit.metadata.status | Authority Work and Publication Information Needed | en_US |
