Show simple item record

dc.contributor.authorJaiswal, Swati
dc.contributor.authorNyquist, Sarah K.
dc.contributor.authorBoyce, Shayla
dc.contributor.authorJivanjee, Tasneem
dc.contributor.authorIbrahim, Samira
dc.contributor.authorBromley, Joshua D.
dc.contributor.authorGatter, G. J.
dc.contributor.authorGideon, Hannah
dc.contributor.authorPatel, Kush
dc.contributor.authorGanchua, Sharie K.
dc.contributor.authorBerger, Bonnie
dc.contributor.authorFortune, Sarah M.
dc.contributor.authorFlynn, JoAnne L.
dc.contributor.authorShalek, Alex K.
dc.contributor.authorBehar, Samuel M.
dc.date.accessioned2022-09-19T13:57:42Z
dc.date.available2022-09-19T13:57:42Z
dc.date.issued2022-09-12
dc.identifier.urihttps://hdl.handle.net/1721.1/145483
dc.description.abstractAbstract Background Cynomolgus macaque (Macaca fascicularis) is an attractive animal model for the study of human disease and is extensively used in biomedical research. Cynomolgus macaques share behavioral, physiological, and genomic traits with humans and recapitulate human disease manifestations not observed in other animal species. To improve the use of the cynomolgus macaque model to investigate immune responses, we defined and characterized the T cell receptor (TCR) repertoire. Result We identified and analyzed the alpha (TRA), beta (TRB), gamma (TRG), and delta (TRD) TCR loci of the cynomolgus macaque. The expressed repertoire was determined using 22 unique lung samples from Mycobacterium tuberculosis infected cynomolgus macaques by single cell RNA sequencing. Expressed TCR alpha (TRAV) and beta (TRBV) variable region genes were enriched and identified using gene specific primers, which allowed their functional status to be determined. Analysis of the primers used for cynomolgus macaque TCR variable region gene enrichment showed they could also be used to amplify rhesus macaque (M. mulatta) variable region genes. Conclusion The genomic organization of the cynomolgus macaque has great similarity with the rhesus macaque and they shared > 90% sequence similarity with the human TCR repertoire. The identification of the TCR repertoire facilitates analysis of T cell immunity in cynomolgus macaques.en_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttps://doi.org/10.1186/s12864-022-08867-0en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceBioMed Centralen_US
dc.titleIdentification and characterization of the T cell receptor (TCR) repertoire of the cynomolgus macaque (Macaca Fascicularis)en_US
dc.typeArticleen_US
dc.identifier.citationBMC Genomics. 2022 Sep 12;23(1):647en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mathematicsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.identifier.mitlicensePUBLISHER_CC
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2022-09-18T03:13:26Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dspace.date.submission2022-09-18T03:13:26Z
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record