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dc.contributor.authorIssigonis, Melanie
dc.contributor.authorRedkar, Akshada B
dc.contributor.authorRozario, Tania
dc.contributor.authorKhan, Umair W
dc.contributor.authorMejia-Sanchez, Rosa
dc.contributor.authorLapan, Sylvain W
dc.contributor.authorReddien, Peter W
dc.contributor.authorNewmark, Phillip A
dc.date.accessioned2023-01-03T17:12:52Z
dc.date.available2023-01-03T17:12:52Z
dc.date.issued2022
dc.identifier.urihttps://hdl.handle.net/1721.1/146952
dc.description.abstract<jats:p>Sexually reproducing animals segregate their germline from their soma. In addition to gamete-producing gonads, planarian and parasitic flatworm reproduction relies on yolk cell–generating accessory reproductive organs (vitellaria) supporting development of yolkless oocytes. Despite the importance of vitellaria for flatworm reproduction (and parasite transmission), little is known about this unique evolutionary innovation. Here, we examine reproductive system development in the planarian <jats:italic>Schmidtea mediterranea</jats:italic>, in which pluripotent stem cells generate both somatic and germ cell lineages. We show that a homolog of the pluripotency factor Klf4 is expressed in primordial germ cells (PGCs), presumptive germline stem cells (GSCs), and yolk cell progenitors. Knockdown of this <jats:italic>klf4-like</jats:italic> (<jats:italic>klf4l</jats:italic>) gene results in animals that fail to specify or maintain germ cells; surprisingly, they also fail to maintain yolk cells. We find that yolk cells display germ cell–like attributes and that vitellaria are structurally analogous to gonads. In addition to identifying a new proliferative cell population in planarians (yolk cell progenitors) and defining its niche, our work provides evidence supporting the hypothesis that flatworm germ cells and yolk cells share a common evolutionary origin.</jats:p>en_US
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)en_US
dc.relation.isversionof10.1371/JOURNAL.PBIO.3001472en_US
dc.rightsCreative Commons Attribution 4.0 International licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourcePLoSen_US
dc.titleA Krüppel-like factor is required for development and regeneration of germline and yolk cells from somatic stem cells in planariansen_US
dc.typeArticleen_US
dc.identifier.citationIssigonis, Melanie, Redkar, Akshada B, Rozario, Tania, Khan, Umair W, Mejia-Sanchez, Rosa et al. 2022. "A Krüppel-like factor is required for development and regeneration of germline and yolk cells from somatic stem cells in planarians." PLoS Biology, 20 (7).
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalPLoS Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2023-01-03T17:08:22Z
dspace.orderedauthorsIssigonis, M; Redkar, AB; Rozario, T; Khan, UW; Mejia-Sanchez, R; Lapan, SW; Reddien, PW; Newmark, PAen_US
dspace.date.submission2023-01-03T17:08:28Z
mit.journal.volume20en_US
mit.journal.issue7en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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