Structural basis of nucleosome retention during transcription elongation
Name
nihms-1836442.pdf
Description
Accepted version
Size
1.25 MB
Format
Adobe PDF
Checksum (MD5)
5ce8471ae3ac089571fd17f715d025f0
Author(s)
Filipovski, Martin
Soffers, Jelly HM
Vos, Seychelle M
Farnung, Lucas
Date Issued
2022
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Filipovski, Martin, Soffers, Jelly HM, Vos, Seychelle M and Farnung, Lucas. 2022. "Structural basis of nucleosome retention during transcription elongation." Science, 376 (6599).
Version
Author's final manuscript
Abstract
In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been unclear. Here, we report the 3.0-angstrom cryo–electron microscopy structure of a mammalian Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within the nucleosome. The structure provides a mechanistic basis for nucleosome retention during transcription elongation where upstream DNA emerging from the Pol II cleft has rewrapped the proximal side of the nucleosome. The structure uncovers a direct role for Pol II and transcription elongation factors in nucleosome retention and explains how nucleosomes are retained to prevent the disruption of chromatin structure across actively transcribed genes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1126/SCIENCE.ABO3851
