In Situ Covalent Functionalization of DNA Origami Virus-like Particles
Name
nihms-1739368.pdf
Description
Accepted version
Size
534.12 KB
Format
Adobe PDF
Checksum (MD5)
9152df27e86eb423a9afbcab372442df
Author(s)
Knappe, Grant A
Wamhoff, Eike-Christian
Read, Benjamin J
Irvine, Darrell J
Bathe, Mark
Date Issued
2021
Journal
ACS Nano
Publisher
American Chemical Society (ACS)
Citation
Knappe, Grant A, Wamhoff, Eike-Christian, Read, Benjamin J, Irvine, Darrell J and Bathe, Mark. 2021. "In Situ Covalent Functionalization of DNA Origami Virus-like Particles." ACS Nano, 15 (9).
Version
Author's final manuscript
Abstract
DNA origami is a powerful nanomaterial for biomedical applications due in part to its capacity for programmable, site-specific functionalization. To realize these applications, scalable and efficient conjugation protocols are needed for diverse moieties ranging from small molecules to biomacromolecules. Currently, there are no facile and general methods for in situ covalent modification and label-free quantification of reaction conversion. Here, we investigate the postassembly functionalization of DNA origami and the subsequent high-performance liquid chromatography-based characterization of these nanomaterials. Following this approach, we developed a versatile DNA origami functionalization and characterization platform. We observed quantitative in situ conversion using widely accessible click chemistry for carbohydrates, small molecules, peptides, polymers, and proteins. This platform should provide broader access to covalently functionalized DNA origami, as illustrated here by PEGylation for passivation and HIV antigen decoration to construct virus-like particle vaccines.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1021/ACSNANO.1C03158
