MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples

Author(s)
Karlikow, Margot; da Silva, Severino Jefferson Ribeiro; Guo, Yuxiu; Cicek, Seray; Krokovsky, Larissa; Homme, Paige; Xiong, Yilin; Xu, Talia; Calderón-Peláez, Maria-Angelica; Camacho-Ortega, Sigrid; Ma, Duo; de Magalhães, Jurandy Júnior Ferraz; Souza, Bárbara Nayane Rosário Fernandes; de Albuquerque Cabral, Diego Guerra; Jaenes, Katariina; Sutyrina, Polina; Ferrante, Tom; Benitez, Andrea Denisse; Nipaz, Victoria; Ponce, Patricio; Rackus, Darius G; Collins, James J; Paiva, Marcelo; Castellanos, Jaime E; Cevallos, Varsovia; Green, Alexander A; Ayres, Constância; Pena, Lindomar; Pardee, Keith; ... Show more Show less
Thumbnail
DownloadPublished version (1.942Mb)
Publisher with Creative Commons License

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0/
Metadata
Show full item record
Abstract
<jats:title>Abstract</jats:title><jats:p>In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access to diagnostic tests. Here we report the results of double-blinded studies of the performance of paper-based diagnostic tests for the Zika and chikungunya viruses in a field setting in Latin America. The tests involved a cell-free expression system relying on isothermal amplification and toehold-switch reactions, a purpose-built portable reader and onboard software for computer vision-enabled image analysis. In patients suspected of infection, the accuracies and sensitivities of the tests for the Zika and chikungunya viruses were, respectively, 98.5% (95% confidence interval, 96.2–99.6%, 268 serum samples) and 98.5% (95% confidence interval, 91.7–100%, 65 serum samples) and approximately 2 aM and 5 fM (both concentrations are within clinically relevant ranges). The analytical specificities and sensitivities of the tests for cultured samples of the viruses were equivalent to those of the real-time quantitative PCR. Cell-free synthetic biology tools and companion hardware can provide de-centralized, high-capacity and low-cost diagnostics for use in low-resource settings.</jats:p>
Date issued
2022
URI
https://hdl.handle.net/1721.1/147789
Department
Massachusetts Institute of Technology. Department of Biological Engineering
Journal
Nature Biomedical Engineering
Publisher
Springer Science and Business Media LLC
Citation
Karlikow, Margot, da Silva, Severino Jefferson Ribeiro, Guo, Yuxiu, Cicek, Seray, Krokovsky, Larissa et al. 2022. "Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples." Nature Biomedical Engineering, 6 (3).
Version: Final published version

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.