Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants
Name
pnas.2111173118.pdf
Description
Published version
Size
705.97 KB
Format
Adobe PDF
Checksum (MD5)
2eb6b647ad866d69ce5f0bd703dfa044
Author(s)
Stopfer, Lauren E
Gajadhar, Aaron S
Patel, Bhavin
Gallien, Sebastien
Frederick, Dennie T
Boland, Genevieve M
Sullivan, Ryan J
White, Forest M
Date Issued
2021
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
Proceedings of the National Academy of Sciences
Citation
Stopfer, Lauren E, Gajadhar, Aaron S, Patel, Bhavin, Gallien, Sebastien, Frederick, Dennie T et al. 2021. "Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants." Proceedings of the National Academy of Sciences of the United States of America, 118 (37).
Version
Final published version
Abstract
Absolute quantification measurements (copies per cell) of peptide major histocompatibility complex (pMHC) antigens are necessary to inform targeted immunotherapy drug design; however, existing methods for absolute quantification have critical limitations. Here, we present a platform termed SureQuant-IsoMHC, utilizing a series of pMHC isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine endogenous pMHC concentrations for a panel of 18 tumor antigens. We apply SureQuant-IsoMHC to measure changes in expression of our target panel in a melanoma cell line treated with a MEK inhibitor and translate this approach to estimate antigen concentrations in melanoma tumor biopsies.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1073/PNAS.2111173118
