dc.contributor.author | Huisman, Brooke D | |
dc.contributor.author | Grace, Beth E | |
dc.contributor.author | Holec, Patrick V | |
dc.contributor.author | Birnbaum, Michael E | |
dc.date.accessioned | 2023-02-21T18:10:26Z | |
dc.date.available | 2023-02-21T18:10:26Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/148130 | |
dc.description.abstract | T cells detect peptide antigens presented by major histocompatibility complex (MHC) proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with trillions of potential peptide-MHC targets, makes it challenging to study, characterize, and manipulate the peptide repertoire of a given TCR. Yeast display has been utilized to study the interactions between peptide-MHCs and T cell receptors to facilitate high-throughput screening of peptide-MHC libraries. Here we present insights on designing and validating a peptide-MHC yeast display construct, designing and constructing peptide libraries, conducting selections, and preparing, processing, and analyzing peptide library sequencing data. Applications for this approach are broad, including characterizing peptide-MHC recognition profiles for a TCR, screening for high-affinity mimotopes of known TCR-binding peptides, and identifying natural ligands of TCRs from expanded T cells. | en_US |
dc.language.iso | en | |
dc.publisher | Springer US | en_US |
dc.relation.isversionof | 10.1007/978-1-0716-2285-8_15 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | Prof. Birnbaum | en_US |
dc.title | Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Huisman, Brooke D, Grace, Beth E, Holec, Patrick V and Birnbaum, Michael E. 2022. "Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors." Methods Mol Biol, 2491. | |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.relation.journal | Methods Mol Biol | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2023-02-21T18:03:23Z | |
dspace.orderedauthors | Huisman, BD; Grace, BE; Holec, PV; Birnbaum, ME | en_US |
dspace.date.submission | 2023-02-21T18:03:24Z | |
mit.journal.volume | 2491 | en_US |
mit.license | OPEN_ACCESS_POLICY | |
mit.metadata.status | Authority Work and Publication Information Needed | en_US |