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dc.contributor.authorCheng, Hoi Lok
dc.contributor.authorJia, Huan
dc.contributor.authorLim, Sing Mei
dc.contributor.authorNg, Say Yong
dc.contributor.authorKongsuphol, Patthara
dc.contributor.authorMcBee, Megan E.
dc.contributor.authorSikes, Hadley D.
dc.date.accessioned2024-04-12T15:46:39Z
dc.date.available2024-04-12T15:46:39Z
dc.date.issued2023
dc.identifier.issn1759-9660
dc.identifier.issn1759-9679
dc.identifier.urihttps://hdl.handle.net/1721.1/154130
dc.description.abstractThe abundance and low production cost of biomaterial cellulose paper have attracted attention for many applications. Point-of-care (PoC) diagnostic tests have been successfully developed using patterned cellulose paper. Although PoC diagnostic tests are rapid and simple to perform, their sample processing throughput is limited, allowing for only one sample to be evaluated at a time, which restricts potential applications. Thus, it was appealing to expand cellulose-based PoC tests to high-throughput versions to increase their applicability. Here, we present the development of a high-throughput cellulose-based 96-well plate vertical flow pull-down assay that can process 96 tests, is easy to prepare, and can be customized for different detection targets. The device has two key features: (i) patterned cellulose paper for 96 tests that do not require pre-immobilization of capturing reagents, and (ii) reusable sturdy housing. We believe that a variety of applications, including laboratory testing, population surveillance tests, and sizable clinical trials for diagnostic tests, can benefit from the adoption of this cellulose-based 96-well plate assay.en_US
dc.description.sponsorshipNational Research Foundation Singapore; Singapore-MIT Alliance for Research and Technology Centreen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionof10.1039/d3ay00614jen_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/en_US
dc.sourceRoyal Society of Chemistryen_US
dc.subjectGeneral Engineeringen_US
dc.subjectGeneral Chemical Engineeringen_US
dc.subjectAnalytical Chemistryen_US
dc.titleDevelopment of a cellulose-based 96-well plate vertical flow pull-down assayen_US
dc.typeArticleen_US
dc.identifier.citationCheng, Hoi Lok, Jia, Huan, Lim, Sing Mei, Ng, Say Yong, Kongsuphol, Patthara et al. 2023. "Development of a cellulose-based 96-well plate vertical flow pull-down assay." Analytical Methods, 15 (28).
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineering
dc.relation.journalAnalytical Methodsen_US
dc.identifier.mitlicensePUBLISHER_CC
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.date.submission2024-04-12T13:56:27Z
mit.journal.volume15en_US
mit.journal.issue28en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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