Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature
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Ohlendorf2024accepted_040524.pdf
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7.26 MB
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Author(s)
Ohlendorf, Robert
Li, Nan
Phi Van, Valerie Doan
Schwalm, Miriam
Ke, Yuting
Dawson, Miranda
Jiang, Ying
Das, Sayani
Stallings, Brenna
Zheng, Wen Ting
Date Issued
May 10, 2024
Journal
Nature Biomedical Engineering
Publisher
Springer Nature
Citation
Ohlendorf, R., Li, N., Phi Van, V.D. et al. Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature. Nat. Biomed. Eng (2024).
Version
Author's final manuscript
Abstract
Bioluminescent probes are widely used to monitor biomedically relevant processes and cellular targets in living animals. However, the absorption and scattering of visible light by tissue drastically limit the depth and resolution of the detection of luminescence. Here we show that bioluminescent sources can be detected with magnetic resonance imaging by leveraging the light-mediated activation of vascular cells expressing a photosensitive bacterial enzyme that causes the conversion of bioluminescent emission into local changes in haemodynamic contrast. In the brains of rats with photosensitized vasculature, we used magnetic resonance imaging to volumetrically map bioluminescent xenografts and cell populations virally transduced to express luciferase. Detecting bioluminescence-induced haemodynamic signals from photosensitized vasculature will extend the applications of bioluminescent probes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Nuclear Science and Engineering
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Creative Commons Attribution-Noncommercial-ShareAlike
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DOI of Published Version
https://doi.org/10.1038/s41551-024-01210-w
