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dc.contributor.authorPluta, Aneta
dc.contributor.authorJaworski, Juan P.
dc.contributor.authorDroscha, Casey
dc.contributor.authorVanderWeele, Sophie
dc.contributor.authorTaxis, Tasia M.
dc.contributor.authorValas, Stephen
dc.contributor.authorBrnić, Dragan
dc.contributor.authorJungić, Andreja
dc.contributor.authorRuano, María J.
dc.contributor.authorSánchez, Azucena
dc.contributor.authorMurakami, Kenji
dc.contributor.authorNakamura, Kurumi
dc.contributor.authorPuentes, Rodrigo
dc.contributor.authorDe Brun, MLaureana
dc.date.accessioned2024-09-03T19:54:03Z
dc.date.available2024-09-03T19:54:03Z
dc.date.issued2024-08-26
dc.identifier.urihttps://hdl.handle.net/1721.1/156536
dc.description.abstractBovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through improved detection and management methods. However, with the increasing novel molecular detection methods there have been few efforts to standardize these results at global scale. This study aimed to determine the interlaboratory accuracy and agreement of 11 molecular tests in detecting BLV. Each qPCR/ddPCR method varied by target gene, primer design, DNA input and chemistries. DNA samples were extracted from blood of BLV-seropositive cattle and lyophilized to grant a better preservation during shipping to all participants around the globe. Twenty nine out of 44 samples were correctly identified by the 11 labs and all methods exhibited a diagnostic sensitivity between 74 and 100%. Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). Finally, the mean correlation value for all assays was within the range of strong correlation. This study highlights the importance of continuous need for standardization and harmonization amongst assays and the different participants. The results underscore the need of an international calibrator to estimate the efficiency (standard curve) of the different assays and improve quantitation accuracy. Additionally, this will inform future participants about the variability associated with emerging chemistries, methods, and technologies used to study BLV. Altogether, by improving tests performance worldwide it will positively aid in the eradication efforts.en_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttps://doi.org/10.1186/s12917-024-04228-zen_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceBioMed Centralen_US
dc.titleInter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samplesen_US
dc.typeArticleen_US
dc.identifier.citationPluta, A., Jaworski, J.P., Droscha, C. et al. Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples. BMC Vet Res 20, 381 (2024).en_US
dc.relation.journalBMC Veterinary Researchen_US
dc.identifier.mitlicensePUBLISHER_CC
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2024-09-01T03:20:51Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dspace.date.submission2024-09-01T03:20:51Z
mit.journal.volume20en_US
mit.licensePUBLISHER_CC
mit.metadata.statusAuthority Work and Publication Information Neededen_US


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