MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Electrostatic microfiltration (EM) enriches and recovers viable microorganisms at low-abundance in large-volume samples and enhances downstream detection

Author(s)
Liu, Yaoping; Raymond, Joshua J; Wu, Xiaolin; Chua, Patrina Wei Lin; Ling, Sharon Yan Han; Chan, Chia Ching; Chan, Cheryl; Loh, Joanne Xin Yi; Song, Melody Xing Yen; Ong, Matilda Yu Yan; Ho, Peiying; Mcbee, Megan E; Springs, Stacy L; Yu, Hanry; Han, Jongyoon; ... Show more Show less
Thumbnail
DownloadPublished version (3.076Mb)
Publisher with Creative Commons License

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution-Noncommercial http://creativecommons.org/licenses/by-nc/3.0/
Metadata
Show full item record
Abstract
Rapid and sensitive detection of pathogens in various samples is crucial for disease diagnosis, environmental surveillance, as well as food and water safety monitoring. However, the low abundance of pathogens (<10 CFU) in large volume (1 mL−1 L) samples containing vast backgrounds critically limits the sensitivity of even the most advanced techniques, such as digital PCR. Therefore, there is a critical need for sample preparation that can enrich low-abundance pathogens from complex and large-volume samples. This study develops an efficient electrostatic microfiltration (EM)-based sample preparation technique capable of processing ultra-large-volume (≥500 mL) samples at high throughput (≥10 mL min−1). This approach achieves a significant enrichment (>8000×) of extremely-low-abundance pathogens (down to level of 0.02 CFU mL−1, i.e., 10 CFU in 500 mL). Furthermore, EM-enabled sample preparation facilitates digital amplification techniques sensitively detecting broad pathogens, including bacteria, fungi, and viruses from various samples, in a rapid (≤3 h) sample-to-result workflow. Notably, the operational ease, portability, and compatibility/integrability with various downstream detection platforms highlight its great potential for widespread applications across diverse settings.
Date issued
2024-09-10
URI
https://hdl.handle.net/1721.1/157512
Department
Singapore-MIT Alliance in Research and Technology (SMART); Massachusetts Institute of Technology. Center for Biomedical Innovation; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Biological Engineering
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry
Citation
Liu, Yaoping, Raymond, Joshua J, Wu, Xiaolin, Chua, Patrina Wei Lin, Ling, Sharon Yan Han et al. 2024. "Electrostatic microfiltration (EM) enriches and recovers viable microorganisms at low-abundance in large-volume samples and enhances downstream detection." Lab on a Chip, 24 (18).
Version: Final published version

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.